碱性成纤维细胞生长因子和转化生长因子β受体在人巩膜成纤维细胞内的表达  

Expression of bFGF receptor and TGF-β receptors in cultured human scleral fibroblasts

作　　者：瞿佳[1] 李翯[1] 周翔天[1] 胡诞宁[1] 张立华[1] 付小莹[1] 吕帆[1] 

机构地区：[1]温州医学院眼视光学院温州医学院近视研究所,325003

出　　处：《中华眼科杂志》2005年第5期464-467,共4页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 ( 30371507 );浙江省自然科学基金资助项目 ( 302095;ZB0202 );浙江省卫生厅重大课题资助项目(2002ZD009);浙江省科技厅科技攻关计划 重点科研项目 -社会发展基金资助项目(2003C23005)

摘　　要：目的　了解人眼巩膜成纤维细胞是否表达碱性成纤维细胞生长因子(bFGF)受体FGFR1和转化生长因子(TGF- β)受体TβRⅠ和TβRⅡ。方法　角膜移植后的4只眼球,应用定点解剖及游走促进法进行巩膜成纤维细胞的分离培养,建立细胞系,采用免疫荧光染色法检测bFGF受体FGFR1和TGF- β受体TβRⅠ和TβRⅡ蛋白的表达。结果　分别应用FGFR 1、TβRⅠ和TβRⅡ的特异多克隆抗体染色,整个细胞表面或细胞核周呈现特异性黄绿色荧光,受体位于细胞胞膜上。肉眼观察TβRⅠ和TβRⅡ呈强阳性表达,FGFR 1表达较弱于TβRⅠ和TβRⅡ。结论　人巩膜成纤维细胞表达bFGF受体FGFR1和TGF β受体TβRⅠ、TβR的功能性蛋白,巩膜是bFGF和TGF- β发挥作用的一个部位。外源的bFGF和TGF -β通过与巩膜成纤维细胞上的上述相应受体结合发挥作用,是影响实验性近视发生发展的机制之一。Objective To examine the expression of basic fibroblast growth factor receptor (FGF R1) and transforming growth factor-β receptors (TGF-β RI and TGF-β RⅡ) in cultured human scleral fibroblasts. Methods Scleral fibroblasts of passages 2-4 were used for the present studies. Polyclonal antibodies against FGF R1, TGF-β RI and TGF-β RⅡ were used to detect the proteins of these receptors. Indirect immunoflurescene staining method (IIF) was used. Results Antibodies for FGF R1, TGF-β RI and TGF-β RⅡ produced specific staining of the entire cell surface, including the cell membrane enveloping cytoplasmic projections; positive staining in some cells was most intensive in the perinuclear region. Immunostaining mainly originated from the cell membranes, indicating that the presence of the receptor proteins on the cell surface. The intensity of staining for TGF-β RI and TGF-β RⅡ was relatively strong, while staining of FGF R1 was relatively weak. The cells treated with PBS instead of primary antibodies did not produce specific staining. Conclusion This study shows that cultured human scleral fibroblasts express the receptor protein for FGF R1, TGF-β RI and TGF-β RⅡ and also indicates that these growth factors may influence these cells. Exogenous bFGF and TGF-β administration may elicit actions through the binding with these receptors in the scleral fibroblasts.

关 键 词：转化生长因子Β受体 碱性成纤维细胞生长因子(bFGF) TGF-β受体 细胞内 bFGF受体 FGFR1 免疫荧光染色法 TβRⅡ TβRⅠ FGFR-1 发挥作用 多克隆抗体 功能性蛋白 实验性近视 分离培养 细胞表面 阳性表达 肉眼观察 细胞表达 

分 类 号：R778.11[医药卫生—眼科]