长距离小载体PCR检测多发性骨髓瘤IgH基因转换区易位  

Long Distance Vectorette PCR for Detecting Translocation of IgH Gene in Switch Regions of Patients with Multiple Myeloma

作　　者：郭垞[1] 侯健[1] 姜华[1] 

机构地区：[1]第二军医大学长征医院血液科,上海200003

出　　处：《中国实验血液学杂志》2005年第3期460-463,共4页Journal of Experimental Hematology

基　　金：上海市百人计划人才基金资助项目(98BR032);全军九五攻关课题基金资助项目(98M082)

摘　　要：免疫球蛋白重链基因(immunoglobulinheavychain,IgH)相关的染色体易位是多发性骨髓瘤(multiplemyeloma,MM)中常见的染色体异常,可能与发病有关,与疾病的预后也有一定关系。为了快速、简便检测IgH基因相关易位的发生并明确易位的伙伴染色体和克隆断裂点,根据易位的断裂点通常位于IgH基因转换区,参考国外专利和文献,建立了长距离小载体PCR方法。结果表明,用该方法检测骨髓瘤细胞株U266,得到3.5kb的PCR产物,两端序列分别与11q和IgH基因α1转换区同源。结论:长距离小载体PCR是一种有效的检测手段,应用该方法对中国人遗传背景的MM患者进行大样本检测,可以发现IgH基因相关易位的发生和分布,有助于解释该遗传背景下的特殊临床表现,阐明发病机制,寻找MM治疗的分子靶点以及对预后的评估。Chromosomal translocations involving immunoglobulin heavy chain (IgH) locus at 14q32 are commonly seen in multiple myeloma (MM), which may involve in pathogenesis and associate with prognosis. To rapidly detect the occurrence of translocation involving IgH locus and the fellow chromosomes and isolate the break points, According to the break points occured in switch regions, patents and references, a long distance vectorette PCR method was developed. The results showed that using this method, a 3.5 kb PCR product from MM cell line U266 was gained, each ends aligned with 11q and IgHα 1 switch region, respectively. It is concluded that LDV-PCR is effective detection technique. Detecting China MM patients by using this method can interpret some specific clinic statues and pathogenesis mechanisms, and help to find new therapy targets and to assess the patient prognosis.

关 键 词：多发性骨髓瘤 免疫球蛋白重链基因 染色体易位 长距离小载体PCR 

分 类 号：R733.3[医药卫生—肿瘤]