人类乳腺癌细胞系Bcap-37和MCF-7染色体核型特征和标记染色体的分析(英文)  

Characteristics of chromosome karyotype and marker chromosome of human breast cancer cell lines Bcap-37 and MCF-7

作　　者：邹俊华[1] 杨君峥[1] 闵凌峰[1] 柳家英[1] 

机构地区：[1]北京大学医学部医学遗传学系,北京市100083

出　　处：《中国临床康复》2005年第18期258-259,i008,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:肿瘤的细胞遗传学研究表明,肿瘤染色体的改变具有非随机性,一些肿瘤还存在有特异的染色体异常,从而为癌基因的表达提供了细胞遗传学基础。目的:对人类乳腺癌细胞系Bcap-37和MCF-7进行染色体G显带分析,研究其核型特征和标记染色体。设计:以细胞为观察对象的对照实验。单位:北京大学医学部医学遗传学系。材料:实验于1991-04/1992-05在北京大学医学部医学遗传学系完成。人类乳腺癌细胞系Bcap-37和MCF-7。方法:应用低温同步法与秋水酰胺处理制备染色体标本,对人类乳腺癌细胞系Bcap-37和MCF-7的中期及早中期细胞进行G-显带分析。对每个细胞系计数50～60个分裂相,分析15～16个G-显带核型,包括320条带和500条带左右水平的分裂相。主要观察指标:两个乳腺癌细胞系染色体数目以及结构改变。结果:Bcap-37细胞染色体众数为63,可识别其结构的标记染色体17条;MCF-7细胞染色体众数为56,可识别其结构的标记染色体13条。结论:两个乳腺癌细胞系均有复杂的染色体异常,乳腺癌中染色体结构及数目的异常,它们可能引起肿瘤相关基因DNA序列重排,也可能导致某些染色体DNA丢失,从而在乳腺癌发生发展中起一定作用。BACKGROUND:Cytogenetic evidence suggests that chromosomal alteration are not randomly occurring events and some malignancies are characterized by specific ch romosome abnormalities,which provides cytogenetic basis for the expression of on cogenes. OBJECTIVE:To investigate the characteristics of chromosomal karyotype and mar ker chromosome of breast cancer cell lines Bcap- 37and MCF- 7 by means of G- banding chromosomal analysis. DESIGN:A controlled experiment with breast cancer cells as observation subjec ts. SETTING:Department of Medical Genetics,Peking University Health Science Cente r. MATERIALS: This study was carried out in the Department of Medical Genetics of Peking University Health Science Center from April 1991 to May 1992 using bre ast cancer cell lines MCF- 7 and Bcap- 37. METHODS:The chromosomes of human breast cancer cell lines Bcap- 37 and MCF- 7 were obtained by growth synchronization induced by hypothermia and colchicine s treatment.The cells at prometaphase or metaphase underwent G- banding chromos omal analysis.For each cell line,50 to 60 mitotic figures were counted and 15 or 16 G- binding karyotypes were analyzed,including the mitotic figure at the lev el of about 320- and 500- band . MAIN OUTCOME MEASURES: Abnormality in chromosome number and structural aberr ation of the two breast cancer cell lines. RESULTS: The modal chromosomal number of Bcap- 37 cell line was 63,of which 17 marker chromosomes had identifiable structure,as compared with 13 out of 56 chromosomes in modal number of MCF- 7 cell line. CONCLUSION: Both of the two breast cancer cell lines have complex cytogeneti c abnormality in the modal number and structure of the chromosomes,which might r esult in the rearrangement of DNA sequence of the cancer- related genes or DNA depletion,so as to play important roles in the pathogenesis and development of b reast cancer.

关 键 词：乳腺肿瘤 细胞系 染色体显带 

分 类 号：R73[医药卫生—肿瘤]