离子交换法分离发酵液中鸟苷和肌苷  

Studies on the separation of guanosine and inosine in fermentation broth by ion exchange

作　　者：刘辉[1] 武改红[1] 王颖[1] 周昌平[1] 路颖[1] 陈宁[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《生物学杂志》2005年第3期41-43,共3页Journal of Biology

基　　金：天津高等学校科技发展基金项目(20031205)

摘　　要：鸟苷发酵液中鸟苷与副产物肌苷的分离对鸟苷工业有重要的意义。对鸟苷和肌苷的分离条件进行研究,得到了最佳分离条件:采用HD-8树脂,树脂床高度为4cm,前120mL采用0.05mol/L盐酸洗脱收集肌苷,120mL至240mL采用0.74mol/LpH5.0醋酸钠缓冲液洗脱收集鸟苷,可以获得很好的效果。It is very important in the guanosine industry that guanosine must be completely separated from byproduct-inosine.The condition of analysis and separation of guanosine and inosine was studied.The optimal condition was finally obtained.The resin was HD-9=8.Its bed height was 4 centimeter.The gradient eluate solution was 0.05mol/L HCL for the first 120mL eluant of insoine and 0.74mol/L NaAc buffer(pH5.0)for the eluant from 120mL to 400mL of guanosine.The method was useful and repeated well.

关 键 词：离子交换法 分离 发酵液 鸟苷 肌苷 

分 类 号：Q525[生物学—生物化学]