木聚糖相对分子质量分布对里氏木霉合成木聚糖酶的影响  

EFFECTS OF MOLECULAR WEIGHT DISTRIBUTION OF XYLAN ON XYLANASE SYNTHESIS BY TRICHODERMA REESEI RUT C-30

作　　者：毛连山[1] 勇强[1] 姚春才[1] 余世袁[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037

出　　处：《林产化学与工业》2005年第2期59-62,共4页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(30070636);南京林业大学科技创新基金(163030005)

摘　　要：以里氏木霉(Trichodermareesei)RutC-30为产酶菌,研究了相对分子质量(Mw)分布不同的木聚糖对木聚糖酶合成的影响。通过SephadexG-100凝胶过滤色谱分级分离发现木聚糖A中低Mw组分较多,木聚糖B中低Mw组分较少,木聚糖C中低Mw组分最少。分别以这3种木聚糖为碳源合成木聚糖酶,最高木聚糖酶活力分别为153.64、120.84和110.84IU/mL,产酶时间分别为60、72和96h。用这3种碳源合成的木聚糖酶酶解粗木聚糖,酶解2h时,产物中低聚木糖分别占总糖的80.70%、68.56%和66.92%。这表明低Mw组分较多的木聚糖不仅有利于促进木聚糖酶的诱导合成,而且有利于促进内切-1,4-木聚糖酶的合成。Effects of molecular weight distribution of xylan on xylanase synthesis by Trichoderma reesei Rut C-30 have been investigated. By Sephadex G-100 column chromatography, it was found that xylan A contained more low- molecular weight components, and xylan B contained less low-molecular weight components, while xylan C contained the least low-molecular weight components. These xylans were used as carbon source for xylanase synthesis,and presented the highest xylanase activity at 153.64, 120.84, 110.84 IU/mL,and culture time were 60, 72, 96 h, respectively . When xylan A was used as the carbon source for xylanase synthesis, xylo-oligosacchrides were 80.70 % of the total sugars in the hydrolysate, which were increased by 17.71 % and 20.59 %,compared to that xylanases synthesized with xylans B and C. It indicated that the more the low-molecular weight components in xylan, the better the xylanases were induced, and the less the culture time was required to obtain high xylanase activity, and low- molecular weight components of xylan were favorable to inducing endo-1,4- β-D -xylanase.

关 键 词：木聚糖 里氏木霉 木聚糖酶 

分 类 号：TQ351.012[化学工程] Q556[生物学—生物化学]