互隔交链孢酚致DNA聚合酶β基因突变的实验研究  

Study on the Mutation of DNA Polymerase β Affected by Alternariol

作　　者：朱涵[1] 何炜[1] 赵国强[1] 王书春[1] 

机构地区：[1]郑州大学基础医学院,河南郑州450052

出　　处：《河南职工医学院学报》2005年第2期77-80,共4页Journal of Henan Medical College For Staff and Workers

摘　　要：目的观察互隔交链孢酚对NIH/3T3细胞中DNA聚合酶β(DNApolβ)基因突变的影响,研究互隔交链孢酚致癌可能的机制。方法NIH/3T3细胞分为两组:①各正常细胞组;②AOH组,AOH浓度为8μmol·L-1。用RTPCR方法从细胞全RNA中扩增出DNA聚合酶β基因cDNA序列,应用TA克隆技术,克隆至pGEMTeasy载体后进行测序,分析序列变化。结果测序后发现正常组DNA聚合酶β基因序列无任何变化,AOH组中228位点发生TC突变(导致39号氨基酸由Tyr变为His),319位点也有TC突变(导致69号氨基酸由IIe变为Thr)。结论AOH可导致NIH/3T3细胞中的DNA聚合酶β发生点突变,推测AOH的致癌作用可能与引发细胞中DNA聚合酶β基因突变有关。Objective To observe the mutation of DNA polymerase β (DNApol β)in NIH/3T3 cell line affected by alternariol(AOH),and study the mechanism of alternariol inducing cancer.[WTHZ]Methods The cells were affected by AOH,and divided into 2 groups:①normal cells;②AOH8 μmol/L.DNApol β gene was amplified through RT-PCR from the cells,then cloned with pGEM-T easy vector and sequenced.[WTHZ]Results The sequence of normal group was normal,but there was 2 point mutations in another group.[WTHZ]Conclusion AOH can induce point mutation of DNApol β gene.The mechanism of AOH inducing cancer relates to the mutation of DNApol β gene affected by AOH.

关 键 词：DNA聚合酶Β 基因突变 交链孢酚 NIH/3T3细胞 实验研究 RT-PCR方法 cDNA序列 克隆技术 序列变化 基因序列 致癌作用 氨基酸 细胞组 RNA 正常组 后发现 IIe 点突变 测序 位点 

分 类 号：R735.1[医药卫生—肿瘤] R734.2[医药卫生—临床医学]