多位点酶电泳法在新疆伤寒沙门氏菌分类和群体遗传学研究中的应用  

Application of Multilocus Enzyme Electrophoresis in Studies of Taxonomy and Population Genetics of S. typhi in Xinjiang

作　　者：杨珊[1] 徐文斌[1] 刘敏生[1] 侯惠珍[1] 刘远恒[1] 祁国明[1] 

机构地区：[1]新疆维吾尔自治区卫生防疫站,中国预防医学科学院流行病学微生物学研究所,中国预防医学科学院

出　　处：《中华流行病学杂志》1994年第1期42-45,共4页Chinese Journal of Epidemiology

摘　　要：采用多位点酶电泳（ＭＥＥ）法对新疆近１１年分离的１５４株伤寒沙门氏菌进行部分多位点醇基因型分类和群体遗传学研究．共测定分析７种代谢酶．研究结果表明，上述菌株共分为６９个电泳型（ＥＴ），分属１０个克隆系（ＣＬａ～ＣＬｊ），其中８０株爆发菌株分属２１个ＥＴｓ，３个ＣＬ（ＣＬｅ、ｆ和ｊ），以ＥＴ１７、２０、２１、２４、２６和２７为主，占６１．３０％，ＣＬｆ为优势克隆系，菌株占９５．００％；７４株散发菌株分属４８个ＥＴｓ，归为９个ＣＬ（ＣＬａ、ｂ、ｃ、ｄ、ｆ、ｇ、ｈ、ｉ、ｊ），没有发现菌株较为集中的ＥＴ，主要克隆ＣＬｈ内菌株占４７．３０％，显示爆发和散发均可由多个ＥＴ型和克隆菌株引起，但爆发荫株的ＥＴ分布更为集中，有明显优势克隆．对上述菌株群体遗传结构分析表明：被测７种代谢酶基因位点均具有多态性，平均杂合度（ｈ）为６．１４，平均遗传多态值（Ｈ）为０．３５．不同来源菌株相比ｈ和Ｈ均无显著性差异（Ｐ均大于０．０５）．表明新疆不同来源的伤寒沙门氏菌可能来自同一群体．结果还显示每个酶基因位点都有一个优势等位基因，提示在伤寒沙门氏菌的进化演变过程中以自然选择为主。Multilocs enzyme electrophoresis( MEE)was employed to study the genotype taxonand population genetics of 154 isolatesof S. typhi from XinJiang in recent 11 years.Seven kinds of enzymes were examined anddetermined. One hundred and fifty-four strainsof S. typhi were classified into 69 electro- retic types(ETs ) and 10 clones(CLa-CLj). Among those 154 strains 80 epidemic isolatesbelonged to 21 ETs and 3 clones(CLe,f,j).ET17,20, 21 , 24,26, 27 were the main ETs(61.30%),and CLf was the dominant clone (95.00%).Seventy-four non-epidemic isolates belonged to 48 ETs and 9 clones(CLa,b,c,d,f,g,h,i,j).no obvious dominant ETwas found in them.and the main clone CLhaccounted for 43.30%. It demonstrated that bothoutbreak and sporadic epidemics could be causedby many strains of ETs and clones, butthe distribution of the former ETs was moreconcentrated and the preponderance of cloneswas more obvious.The analyses on structnreof popnlation genetics indicated thatall of 7 enzymes were polymorphic. Geneticdiversity among the enzyme loci was quite high.with an average of 0.35 per locus (H).Tht average mumber of alleles per locus(h)was 6.14.There was no significantdifference in h or H between the isolates ofdifferent sources(P> 0.05).It was clear thatS.typhi of different sources in Xinjiang wereoriginated from the same popnlation. Theresults also showed that there was a dominantallele in every enzyme locus.Natural selectionwas the main role in the evolution of S.typhi.

关 键 词：多位点酶电泳 伤寒沙门氏菌 遗传学 

分 类 号：R378.23[医药卫生—病原生物学]