选择性计数双歧杆菌培养基的效果比较  

The Comparison of Selective Enumeration Medium of Bifidobacterium

作　　者：孟祥晨[1] 庞睿[2] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室 [2]东北农业大学食品学院,哈尔滨150030

出　　处：《食品与发酵工业》2005年第5期1-4,共4页Food and Fermentation Industries

基　　金：黑龙江省自然科学基金重点项目(ZJN03-3);黑龙江省教育厅重大项目(105117003)

摘　　要：随着双歧杆菌基础研究和开发应用的迅速发展,双歧杆菌活菌计数方法越来越重要,实验目的是要选择1种适于选择性计数产品中双歧杆菌菌落数的培养基。主要采用菌落技术方法,比较了常用的8种选择性培养基对7种产品中双歧杆菌的选择性计数效果。结果表明,以改良MRS-X-Gal琼脂培养基上形成的双歧杆菌菌落数最高,且菌落特征明显,易于计数,是优选出的效果较好的选择性计数培养基。With the rapid development of basic research and application of Bifidobacterium in food,the method of selective enumeration of Bifidobacterium becomes very important. The aim of this study is to select a medium that can selectively enumerate Bifidobacterium in food. The comparison of selective enumeration for eight selective media was studied with colony enumeration method. The results showed that the bifidobacteria colony formed on modified MRS-X-Gal were highest among eight selective media, and the characteristics of colonies were obvious. Modified MRS-X-Gal was best for selective enumeration of Bifidobacterium among eight selective media.

关 键 词：选择性计数 双歧杆菌 效果比较 选择性培养基 开发应用 基础研究 计数方法 实验目的 技术方法 菌落特征 菌落数 产品 活菌 琼脂 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]