微生物絮凝剂产生菌的培养条件研究  

Study on culture conditions of a bio-flocculant-producing strain

作　　者：张平[1] 戴友芝[2] 唐受印[3] 

机构地区：[1]中南林学院资源与环境学院,长沙410004 [2]湘潭大学环境工程系,湘潭411105 [3]湖南工程学院,湘潭411104

出　　处：《环境污染治理技术与设备》2005年第4期25-27,共3页Techniques and Equipment for Environmental Pollution Control

基　　金：湖南省计委资助项目(湘计高技[2001]907号)

摘　　要：从活性污泥中分离筛选到1株高效絮凝剂产生菌E-9,初步鉴定其为大肠杆菌.首次发现大肠杆菌能产生微生物絮凝剂.该菌产生絮凝剂的适宜培养条件为:乙醇为碳源,大豆粉为氮源,初始pH值为6.5～7.2、温度为30℃.絮凝实验结果表明,该菌产絮凝剂对高岭土悬浊液的絮凝率可达97%～99.5%,而絮凝剂投加量仅为通用发酵培养基的1/15,絮凝效果优于聚丙烯酰胺等常用絮凝剂.A bacterial strain designated as E.coli and named E-9 which can produce bio-flocculant with high flocculanting activity was screened from activated sludge .It is the first time to find that E.coli producing bioflocculant. The optimum culture conditions:alcohol is the optimum carbon source, soybean powder is the optimum nitrogen source, initial pH 6.5~7.2, 30 ℃.The results of flocculation test show that the E-9 can produce a strong-activity flocculants, which has higher flocculating effect compared with other ordinary flocculants, with flocculating rate of 97%~99.5% against kaolin clay suspension, and its dose is about 1/15 of the ferment media.

关 键 词：微生物絮凝剂产生菌 培养条件 大肠杆菌 初始PH值 发酵培养基 聚丙烯酰胺 分离筛选 活性污泥 初步鉴定 首次发现 絮凝实验 絮凝效果 大豆粉 絮凝率 悬浊液 高岭土 投加量 碳源 

分 类 号：TQ925[轻工技术与工程—发酵工程] TU991.22[建筑科学—市政工程]