高选择性重组菌在含Hg^(2+)环境中的生长富集Hg^(2+)耦合及其连续处理Hg^(2+)废水  

Simultaneous Growth and Bioaccumulation of Mercuric Ion by Genetically Engineered E.coli and Its Continuous Treatment

作　　者：郑杨春[1] 邓旭[1] 李清彪[1] 卢英华[1] 何宁[1] 孙道华[1] 彭雅娟[1] 

机构地区：[1]厦门大学化学工程与生物工程系,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第B06期98-101,共4页Journal of Xiamen University：Natural Science

基　　金：教育部优秀青年教师资助计划项目;福建省自然科学基金(D0310004)资助

摘　　要：研究了在低营养含汞环境中,高选择性汞富集基因工程菌E.coliJM109(表达MerT、MerP汞特异结合转运蛋白和金属硫蛋白MT,该菌简称为M1)的生长富集耦合行为.考察了汞离子浓度、离子强度、络合物、共存金属离子等环境因素对其生长富集Hg2+耦合行为的影响;并且利用连续操作搅拌槽式生物反应器,实现了对有机废水配伍的含汞废水的连续化处理.Genetically engineered E.coli JM109,namely M1,which expressed both Hg^2+ transport system and metallothionein,was tested for its capability of simultaneous growth and bioaccumulation of Hg^2+ (SG&B) under low nutritional circumstances.The influential factors of ambient conditions,e.g.initial concentrations of mercuric ion,ionic strength, the presence of metal chelators and other coexisting metal ions were investigated.Hg^2+ bioaccumulation behavior of M1 was proved to be well coupled with its growth of M1.Furthermore,continuous treatment of Hg^2+ contaminated organic wastewater was realized via continuous stirred-tank bioreactor.

关 键 词：HG^2+ 高选择性 富集 连续处理 耦合 生长 重组菌 E.coli 基因工程菌 金属硫蛋白 生物反应器 转运蛋白 特异结合 离子浓度 离子强度 环境因素 金属离子 连续操作 含汞废水 有机废水 络合物 连续化 

分 类 号：X131.2[环境科学与工程—环境科学] TQ241.12[化学工程—有机化工]