抗人肝癌单克隆抗体轻链可变区基因的体外扩增、克隆及序列测定  

SEQUENCE OF LIGHT CHAIN VARIABLE REGION GENE OF A MONOCLONAL ANTTBODY TO HUMAN HEPATOCARCINOMA

作　　者：杨萍[1] 刘彦仿[1] 胡川闽[1] 陈志南[1] 隋延仿[1] 陈苏民[1] 高磊[1] 陈南春[1] 杨安钢[1] 

机构地区：[1]第四军医大学病理教研室

出　　处：《中华肿瘤杂志》1994年第4期263-265,共3页Chinese Journal of Oncology

基　　金：国家"863"资助课题

摘　　要：我们应用一对引物利用聚合酶链反应（ＰＣＲ）技术从小鼠抗人肝癌单克隆抗体杂交瘤细胞株ＨＡｂ２７ＲＮＡ中扩增出了抗体轻链可变区基因，将此基因重组λＭ１３噬菌体ＤＮＡ中，测定了此可变区基因的全序列，经计算机分析，结果表明基因长度为３２４ｂｐ编码１０８个氨基酸，是具有功能性的抗体轻链可变区基因，在核苷酸序列上与鼠胚系基因ＶｋＯＸ１具有８５％同源性，所得基因为Ｖｋ与Ｊｋ４重排。Abstract The isolation of the,rearranged immunoglobulin genes from a hybridoma cell line,which is a prerequisite for the construction of a recombinant antibody, can easily be achieved by PCR Wehave ,determined the mucleotide sequence of the variable region of L chain of a high affinity monoclonal antibody HAb2w7 directed to human hepatocarcinoma Analysis of the mucleotide se -quence of the light chain V region of HAb27 revealed that the Vksequence was 85 %identical to germline VkOxl sequence.

关 键 词：肝肿瘤 单克隆抗体 聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]