大蒜素诱导人膀胱癌细胞株BIU87细胞凋亡的研究  

Apoptosis of BIU87 Cells Induced by Allitridi

作　　者：曹开源[1] 毛晓鹏[2] 丘少鹏[2] 袁广卿[3] 陈显景[2] 徐霖[4] 黄晓荣[3] 

机构地区：[1]中山大学检验医学系,广东广州510080 [2]中山大学附属第一医院泌尿外科,广东广州510080 [3]中山大学基础医学院教学中心,广东广州510080 [4]中山大学基础医学院免疫教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2005年第4期432-434,438,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金面上项目(010719)

摘　　要：【目的】探讨大蒜素对人膀胱癌细胞株BIU87细胞凋亡的影响。【方法】MTT法检测大蒜素对BIU87细胞生长的影响;倒置显微镜、TUNEL法观察细胞形态变化,流式细胞仪检测细胞凋亡率和细胞周期的变化;半定量RT-PCR法检测凋亡相关基因bcl-2,bax,caspase-3mRNA表达的变化,比色法测定caspase-3活性变化。【结果】大蒜素能明显抑制BIU87细胞的生长,与大蒜素的浓度和作用时间有关,5mg/L大蒜素作用5d可以杀死50%以上的细胞;可诱导BIU87细胞凋亡,细胞周期阻滞在S-和G2期;凋亡相关基因bcl-2表达减少,bax无明显变化,caspase-3表达及活性增高。【结论】诱导细胞凋亡是大蒜素发挥抗瘤效果的一种重要机制;凋亡发生可能与细胞周期阻滞、bcl-2和caspase-3基因表达及活性变化有关。[Objective] To study the mechanism of allitridi-induced apoptosis in BIU87 cells.[Methods] Growth inhibition were estimated using MTT assay. Apoptotic cells were detected by in-situ cell apoptosis detection kit, and confirmed by flow cytometry. The mRNA level affected by allitridi was determined by RT-PCR. Caspase-3 activity was estimated with chromometry. [Results] Allitridi inhibited the proliferation of BIU87 cells and induced apoptosis, S-phase and G2 -phase arrest was induced. There were apparent changes of bcl-2, caspase-3 mRNA and a rise of caspase-3 activity; while no changes of bax was found. [Conclusion]Allitridi can inhibit the proliferation of BIU87 cells by inducting apoptosis, which may be through S-and G2-phase arrest, down-regulation of bcl-2, and increasing caspase-3 expression and its activity.

关 键 词：人膀胱癌细胞株 BIU87 大蒜素 caspase-3 基因BCL-2 细胞周期阻滞 流式细胞仪检测 MTT法检测 细胞形态变化 TUNEL法 PCR法检测 mRNA表达 诱导细胞凋亡 凋亡相关 活性变化 倒置显微镜 细胞凋亡率 比色法测定 基因表达及 

分 类 号：R737.14[医药卫生—肿瘤] R285.5[医药卫生—临床医学]