同位素稀释GC-LRMS法测定鱼肉中指示性多氯联苯方法研究  

Study on determination of polycholorinated biphenyls in fish by isotopedilution GC-LRMS

作　　者：李敬光[1] 吴永宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2005年第3期322-325,共4页Journal of Hygiene Research

基　　金：国家重点基础研究发展规划项目 (No .2 0 0 3CB41 50 0 1 ) ;国家"十五"重大科技专项 (No .2 0 0 1BA80 4A1 3与No .2 0 0 2BA80 4A1 9) ;国家高新技术项目国际合作重点项目 (No.2 0 0 2AA2 1 70 31 )

摘　　要：目的　建立一套同时准确测定食品中的2 0种指示性PCB单体的GC -LRMS方法。方法　样品经过索氏提取,混合硅胶柱,氧化铝柱净化,浓缩后采用HRGC LRMS和同位素稀释定量技术,以选择离子监测方式测定目标化合物。结果　方法的检测限为0 .0 0 9～0 .0 4 4ng g,六个加标鱼样(添加水平1ng g)中2 0个单体的回收率为79 .7%～112 .6 % ,RSD1%～8%。内标回收率在5 0 .0 %～90 . 4 %之间。结论　本方法定量准确可靠,可用于食品中指示性PCB的测定。Objective To develop a method for determination of 20 indicator polycholorinated biphenyls in fish by GC-LRMS.Methods Fish sample was added 13C lablled internal standard and cleaned-up by a multilayer silica column and a alumina column. Sample was analyzed by HRGC-LRMS using SIM mode.Results Detection limits of 20 indicator polycholorinated biphenyls were 0.009-0.044 ng/g.Average recovery were 79.7%-112.6%,RSD were 1%-8%(n=6).Conclusion Results showed that the method was suitable for determination of indicator PCB in fish sample.

关 键 词：多氟联苯 同位素稀释 GC—LRMS 

分 类 号：R155.55[医药卫生—营养与食品卫生学] TS207.5[医药卫生—公共卫生与预防医学]