牛磺酸对东莨菪碱所致记忆获得性障碍模型小鼠的改善作用  

Effect of Taurine on learning-memory ability and M-cholinergic receptor in dementia model mice induced by scopolamine

作　　者：叶翠飞[1] 张兰[1] 张丽[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药理研究室,北京脑老化重点实验室,北京100053

出　　处：《中国药理学通报》2005年第6期715-717,共3页Chinese Pharmacological Bulletin

基　　金：北京市科技新星计划项目(NoH020821390190);首都医学发展基金项目(No20023002);北京市自然科学基金项目(No7032013);国家重点基础研究发展规划(973计划)项目(NoG2000057010);北京市科委资助北京市重点科技实验室项目(No951890600);北京市中医药重点学科项目(2001)

摘　　要：目的观察牛磺酸对东莨菪碱所致模型小鼠学习记忆及对其脑组织M胆碱受体结合力的影响。方法将昆明小鼠随机分组,设立正常对照组,东莨菪碱模型组及阳性对照药(吡拉西坦)组,牛磺酸小剂量组(0.3g·kg-1),中剂量组(0.75g·kg-1),大剂量(1.875g·kg-1)组,分别灌胃给药,于d5,除正常对照组腹腔注射生理盐水外,其它各组注射东莨菪碱1mg·kg-1,20min后进行Morris水迷宫测试,测试5d后处死,取其脑组织进行M受体结合力测定。结果在行为学实验中,牛磺酸各剂量组游出时间与模型组相比有明显缩短(P<0.05),并可以提高模型小鼠M胆碱受体的结合力。结论牛磺酸对东莨菪碱所致模型小鼠学习记忆障碍及M受体有一定的改善作用。Aim To observe the effect of taurine on learning-memory ability and its mechanism in model mice induced by scopolamine(Scop). Methods Kunming mice were randomly divided into normal control group, Scop model group, taurine groups (0.3, 0.75, 1.875 g·kg -1) and Piracetam group (positive control group). The drugs were continually intra-gastrically administrated for 5 days. On day 5, after intraperitoneal injection of scopolamine, mice were subjected to Morris water maze test. 5 days later, M-cholinregic receptor binding was assayed by radio ligand binding test.Results Compared with model group, significant decrease of swimming time and distance in Morris water maze test was observed in all 3 taurine groups. Taurine at middle dose increased M-cholinergic receptor binding in the brain of model mice. Conclusion Taurine effectively improves learning-memory ability in model mice induced by scopolamine injection. The mechanism relates with the improvement of M-cholinergic receptors of brain.

关 键 词：牛磺酸 学习记忆 M胆碱受体 东莨菪碱 

分 类 号：R-332[医药卫生] R322.81