抗肿瘤药物敏感性实验方法的比较  

Comparison of anti-tumor drugs chemosensitivity assays

作　　者：姚静[1] 李小兰[1] 李伟华[1] 吴剑宏[1] 谢大兴[1] 龚建平[1] 

机构地区：[1]华中科技大学同济医学院同济医院分子医学中心肿瘤研究所,武汉430030

出　　处：《中华实验外科杂志》2005年第7期792-793,共2页Chinese Journal of Experimental Surgery

基　　金：卫生部临床重点项目(20012537);973肿瘤计划资助项目(G1998051213)

摘　　要：目的探讨流式细胞术检测抗肿瘤药物敏感性在临床应用的可行性,为新的抗肿瘤药物敏感性检测方法的建立,特异性抗肿瘤药物的筛查提供试验理论基础。方法应用流式细胞凋亡检测技术的SubG1法和AnnexinV法,检测10例Molt4细胞、32例临床胃肠肿瘤细胞在抗肿瘤药物作用下,不同时间点肿瘤细胞的凋亡率。并与噻唑蓝比色(MTT)法进行比较分析。结果AnnexinV法、SubG1法、MTT比色法均可检测到药物对Molt4细胞、临床胃肠肿瘤细胞杀伤率随药物作用时间延长而增加。AnnexinV法、SubG1法、MTT比色法检测药物致细胞最大杀伤率差异无统计学意义(P>0.05)。AnnexinV法检测到药物最大杀伤率的有效时间点明显早于SubG1法MTT比色法,SubG1法检测的有效时间点略早于MTT比色法。结论流式细胞术的AnnexinV法和SubG1法可作为肿瘤药物敏感性有效的检测方法。AnnexinV法比经典MTT比色法具有更敏感、快速、简洁、可靠等优点,有临床应用价值。Objective To investigate the application of flow cytometry in the assessment of anti-tumor drugs chemosensitivity.[WT5”HZ]Methods Sub-G_1 and Annexin V assays were used to detect the apoptosis of Molt-4 cell line and fresh gastrointestinal cancer cells which were exposed to anti-tumor drugs,such as 5-fluorouracil and Paclitaxel.The outcomes were compared with the ones from MTT colormetric assay.Results[WT5”BZ] The outcome of Annexin V assay or Sub-G_1 assay was closely associated with MTT assay.But the time point of the maximum cytotoxicity of drugs detected by Annexin V assay was earlier than that by MTT assay or by Sub-G_1 assay.Conclusion Annexin V and Sub-G_1 assays could be used as the effective method to test the anti-tumro drugs chemosensitivity.Annexin V assay would be a sensitive,rapid,reliable approach for measuring anti-tumor drugs chemosensitivity.

关 键 词：药物敏感性 实验方法 ANNEXIN Sub-G1法 MOLT-4细胞 MTT比色法 流式细胞术检测 抗肿瘤药物作用 肿瘤细胞杀伤率 有效时间点 临床应用价值 检测方法 理论基础 检测技术 细胞凋亡 不同时间 比较分析 时间延长 特异性 凋亡率 

分 类 号：R73-3[医药卫生—肿瘤]