血液链球菌细菌素的提取及其对牙周致病菌的抑制作用  

Bacteriocin Extracted from Streptococcus Sanguis and Its Effect on Suspected Periodontal Pathogens

作　　者：胡琳[1] 张举之[1] 杨美薷[1] 唐光庆[1] 郑镇西 吴泓舟 

机构地区：[1]华西医科大学口腔医学院,卫生部成都生物制品研究所

出　　处：《华西口腔医学杂志》1994年第4期275-278,T016,共5页West China Journal of Stomatology

摘　　要：通过超声破碎、盐析和凝胶过滤从血液链球菌细胞内提取、分离和纯化出细菌素。所提取的血液链球菌细菌素对热处理和蛋白酶敏感，加热６０℃或用蛋白酶、胰蛋白酶处理后其活性完全丧失，对ＤＮＡ酶和ＲＮＡ酶不敏感。在体外，血液链球菌细菌素对许多与牙周组织破坏关系密切的细菌如放线共生放线杆菌、牙龈紫质单胞菌、中间普氏菌、产黑色素普氏菌、具核梭杆菌和二氧化碳噬纤维菌均有抑制作用。经鉴定证明这些作用不是噬菌体和酶的作用，而是细菌素的作用。Abstract In this study,authors observed that S,sanguis produces bacteriocin accumulating intracellularly.It was extracted by ultrasonic treatment and purified by fractionation with ammonium sulfate and gel filtration. The hacteriocin of S.sanguis is resistant to deoxyribonuclease and ribonuclease but is sensitive to pronase and trypsin. The biological activity is lost by heating at 60℃ or by pronase and trypsin. The bacteriocin of S. sanguis which is readily distinguishable from bacteriophage and proteolytic enzyme can inhibit the growth of periodontal pathogens such as A. actinomycetemcomitans,P. gingivalis,P.intermedius,P. melaninogenicus, F. nucleatum,C. gingivalis and C. sputigena in vitro.These results demonstrate that this bacteriocin of S. sanguis is one of the factors that making S. sanguis antagonize the periodontal pathogens.

关 键 词：血液 链球菌 细菌素 牙周病 

分 类 号：R781.405[医药卫生—口腔医学]