Construction of eukaryotic expression vector with brain-derived neurotrophic factor receptor trkB gene  

作　　者：黄涛 姜晓丹 许忠 袁军 丁涟沭 邹雨汐 徐如祥 

机构地区：[1]Department of Neurosurgery, Zhujiang Hospital, Nanfang Medical University, Guangzhou 510282, China

出　　处：《Chinese Journal of Traumatology》2005年第3期142-146,共5页中华创伤杂志（英文版）

基　　金：ThisworkwassupportedbyProgramofSci tech DevelopmentPlansofGuangdongProvince(2003A3020304) andtheKeyProjectFoundationofthe"TenthFive yearPlan"of PLA(01Z054)

摘　　要：Objective: To construct an eukaryotic expression vector carrying rat brain-derived neurotrophic factor receptor trkB gene. Methods: Using the total RNA isolated from rat brain as template, the trkB gene was amplified by reverse-transcription-polymerase chain reaction (RT-PCR) with a pair of specific primers which contained the restrictive sites of EcoR I and BamH I. The amplified fragment of trkB gene was digested with EcoR I and BamH I, and then subcloned into cloning vector pMD18-T and expression vector pEGFP-C2 respectively. The recombinant plasmids were identified by restriction endonuclease enzyme analysis and PCR. Results: The amplified DNA fragment was about 1461 bp in length. Enzyme digestion and PCR analysis showed that the gene of trkB had been successfully cloned into vector pMD18-T and pEGFP-C2. Conclusions: The trkB gene of rat has been amplified and cloned into the eukaryotic expression vector pEGFP-C2.

关 键 词：Neurotrophic factors RECEPTOR Gene rearrangement CLONING 

分 类 号：R346[医药卫生—基础医学]