高效液相色谱法测定阿立哌唑的含量  

HPLC Determination the Content of Aripiprazole

作　　者：蒋晔[1] 田书霞[1] 贾湘曼[1] 蔡太梅[1] 谢赞[1] 

机构地区：[1]河北医科大学药学院,石家庄050017

出　　处：《药物分析杂志》2005年第7期835-837,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法测定阿立哌唑原料药的含量。方法:采用Hypersil C18色谱柱(150 mm×4.6 mm,5μm),流动相:甲醇-磷酸盐缓冲溶液(20 mmol·L-1的磷酸二氢钾溶液,pH 6.3)(62:38),流速:1.0 mL·min-1,检测波长:257 nm。结果:阿立哌唑在0.1-20μg范围内线性关系良好(r=0.9999),检测限5×10-3μg。阿立哌唑与其有关物质的分离良好。结论:该法专属性强,结果准确,重现性好,可用作阿立哌唑含量测定和有关物质检查的方法。Objective:To establish an HPLC method for determination the content of aripiprazole. Method:A Hypersil C18column(150mm×4.6 mm,5μm) was used with the mobile phase of methanol - phosphate buffer solution (pH6.3) (62:38). The flow rate was 1.0mL·min-1 and the detection wavelength was 257 nm. Result:The calibration curves were linear between 0.1-20μg(r=0.9999),the detection limit was 5×10-3μg. Conclusion:The method is simple,rapid,accurate and suitable for determination the content of aripiprazole.

关 键 词：高效液相色谱法 阿立哌唑 磷酸盐缓冲溶液 C18色谱柱 有关物质检查 磷酸二氢钾 检测波长 线性关系 含量测定 原料药 流动相 检测限 专属性 重现性 

分 类 号：R286.0[医药卫生—中药学] R971.4[医药卫生—中医学]