刺五加多糖的提取及含量测定  

Extraction of polysaccharides from Acanthopanax senticosus and determination of polysaccharides content

作　　者：孟凡欣[1] 蒋朝军[1] 于笑坤[1] 徐睦云[1] 逯家辉[1] 刘宏扬[2] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012 [2]中国石油吉林石化分公司,吉林132022

出　　处：《食品工业科技》2005年第7期110-112,共3页Science and Technology of Food Industry

基　　金：吉林省科技发展基金(20020503-2)

摘　　要：探讨了经80℃热水提取、Sevag法除蛋白、乙醇分级沉淀等步骤提取刺五加多糖,并应用苯酚-硫酸法测定其含量。本法选取490nm为反应产物的测定波长,所测葡萄糖标准曲线相关系数r=0.99956,线性范围为5-22.5μg/mL,测得多糖的总收率为刺五加干重的0.54%。本法反应产物在160min内吸光度值稳定,RSD为1.22%,精密度良好,回收率为97.6%-102.8%。This paper studies the extraction and analysis of polysaccharides from Acanthopanax senticosus, the processing steps are as follows: extract the polysaccharides from Acanthopanax senticosus by 80℃ water at first, then deproteinize according to the method of Sevag, and then precipitate the polysaccharide by ethanol. Phenol-sulfuric acid assay is applied to determinate the polysaccharides content quantitatively. In this method, we adopt 490nm as the optimum wavelength for determination, the relative coefficient of the standard curve of glucose is 0.99956, the linear range is 5-22.5 μg/mL The total yield is 0.54% of the desiccated Acanthopanax senticosus. The absorbance of the product doesn't change in 160 minutes, i.e. product is quit stable. The precision is good, the RSD is not more than 1.22%, and the range of recovery is 97.6% -102.8%.

关 键 词：苯酚-硫酸法 刺五加 多糖 提取 

分 类 号：TS201.1[轻工技术与工程—食品科学]