化疗后卵巢癌组织中线粒体DNA的变化  

Alterations of mitochondrial DNA in ovarian cancer patients with and without chemotherapy

作　　者：史宏晖[1] 潘凌亚[1] 杨秀玉[1] 原野[2] 于晓丹[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科 [2]军事医学科学院细胞生物学研究室

出　　处：《中华妇产科杂志》2005年第7期469-471,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(30271357)

摘　　要：目的探讨化疗后卵巢癌组织中线粒体DNA的变化。方法收集2002年11月至2003年8月北京协和医院收治的7例初治和9例化疗后复发的卵巢癌患者的肿瘤组织及其周围的正常组织标本,对其线粒体DNA全长测序,与基因库中数据对比,确定基因库中未记载的新的多态性变化位点和突变位点。结果共发现69个新的多态性变化位点和17个突变位点,多态性变化在初治和复发卵巢癌患者的肿瘤组织和周围的正常组织中均存在,而突变仅在其肿瘤组织中存在。初治标本多态性变化位点平均2.7个,复发标本平均5.6个,两者比较,差异有统计学意义(P=0.01)。初治标本中编码蛋白的多态性变化位点为14个,其中3个(21%)引起了氨基酸的改变;复发标本中编码蛋白的多态性变化位点为41个,其中13个(32%)引起了氨基酸的改变,两者氨基酸改变的发生率比较,差异无统计学意义(P=0.700)。初治标本的突变率为5/7,突变位点有7个,其中处于蛋白编码区的4个突变位点中有3个(3/4)引起了氨基酸的改变;复发标本的突变率为5/9,突变位点有10个,其中处于蛋白编码区的5个突变位点全部(5/5)使氨基酸发生了改变,两者突变率和突变位点引起的氨基酸改变发生率比较,差异均无统计学意义(P>0.05)。结论化疗后复发的卵巢癌组织中,线粒体DNA的多态性变化明显增加,而线粒体DNA的突变无明显增加。提示线粒体DNA突变可能是肿瘤固有的特性之一。Objective To explore the alterations of mitochondrial DNA in ovarian cancer patients with and without chemotherapy. Methods Tumor tissues and their adjacent normal tissues of 9 ovarian cancer patients with chemotherapy and 7 ovarian cancer patients without chemotherapy were collected respectively from November 2002 to August 2003 in Peking Union Medical College Hospital. The complete mitochondrial DNAs were sequenced and the results were compared with the data in Genbank. Results A total of 69 novel polymorphisms and 17 mutations were detected. The mean polymorphisms in patients without chemotherapy were 2.7, whereas those with chemotherapy were 5.6. There was significant difference between them(P=0.01).In the coding area,the proportion of amino acid changes accounted for 21% and 32% in the two groups induced by polymorphisms. The mutation rates were 5/7 and 5/9 in the two groups respectively and the amino acid changes were 3/4 and 5/5. Conclusions There are more polymorphisms which do not alter the amino acids accordingly in ovarian cancer patients with chemotherapy compared with patients without chemotherapy. Meanwhile, the amount of mutations does not enhance in these patients. It indicates that mitochondrial DNA mutation may be a unique characteristic of tumors.

关 键 词：卵巢癌组织 化疗后 2002年11月 线粒体DNA突变 突变位点 2003年8月 卵巢癌患者 肿瘤组织 北京协和医院 编码蛋白 多态性 氨基酸 统计学 突变率 组织标本 正常组织 基因库 编码区 复发 初治 周围 平均 率比 

分 类 号：R737.31[医药卫生—肿瘤] R730.53[医药卫生—临床医学]