肺癌K－ras基因突变的检测及临床应用  

Detectiou of K-ras oncogene activation in human lungcancer and its possible clinical application.

作　　者：张广宇[1] 孙以瑜[1] 王孟山[1] 朱春贤[1] 

机构地区：[1]河北省胸科医院,河北医学院第二附属医院

出　　处：《中华结核和呼吸杂志》1995年第5期282-284,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：为探讨基因检测在肺癌中的应用价值，应用ＰＣＲ－ＲＦＬＰ法检测１０２例肺癌患者手术标本中Ｋ－ｒａｓ基因第１２密码子突变的状况。并将肺腺癌按临床病理特征分组进行对比分析。结果显示，肺癌中总突变率为２４％，腺癌中为４９％，鳞癌为１５％，而小细胞癌中无一例突变。吸烟是促发Ｋ－ｒａｓ基因突变的重要因素（Ｐ＜０．０５），肺腺癌突变组淋巴结转移早、部位远，其一年复发率达８５％，明显高于未突变组（Ｐ＜０．０１）。Ｋ－ｒａｓ基因突变的检测具有重要的临床意义和应用价值。y using a modified polymerase chain reaction(PCR) and restriction fragment length polymorphisrn(RFLP) technique, we detected Kras codon 12 muta-tion in 102 formalin-fixed, paraffinernbedded tissuesfrom surgical samples of lung cancer patients. The X￣2test was used to determine the statistical significance ofdifference, according to the presence or absence of mutation in codon 12 of K-ras oncogene. We found 25 cas-es (24%) positive for mutation of K-ras 12 codon. Mu-tation occured in 6 of the 40 cases (15 %) of squamouscell carcinoma , 18 of 37 adenocarcinoma cases (49%) .1 of 2 adenosquamous cases, 0 of 1 carcinoid patienl ,but no Kras activation was found in small cell carcino-ma (0/22) cases.Analysis of the clinical and pathological features of37 adenocarcinoma cases showed no apparent associa-tions between the Kras codon 12 mutation and sex . dis-ease stage. tumor size (T) , metastatic status (M) andthe degree of differentiation (all P values greater than0.005) . but the nodes (N) of K-ras-positive adenocarci-noma tended to be more than the K-ras-negative ones(P<0. 01) .From 26 male cases of adenocarcinoma mutation incodon 12 of K-ras occur more frequently in adenocarci-noma from smokers than non-smokers (P<0. 05) , sug-gesting that smoking is an important factor in the induc-tion of the mutation.Among 37 adenocarcinoma cases, only 25 cases canbe traced the reeurrence rate in lyear. The 1-year re-currence rates were 85%(11/13) in K-ras mutationalpatients, more than 25%(3/12) in K-ras negative ones(P<0.01) . whereas there was no relationship betweenrecurrence and differentiation in these 25 cases. Thefindings suggest the K-ras gene mutation may be one ofthe prognostic markers for human lung adenocarcinoma.

关 键 词：肺肿瘤 K-RAS基因 基因突变 聚合酶链反应 

分 类 号：R734.202[医药卫生—肿瘤]