杂色曲霉素处理后体外培养的人胚肺细胞增殖情况及p53和rasp21蛋白表达的变化  

Cell Proliferation,p53 and Ras p21 Expression in Human Fetal Lung Cells in Vitro after Sterigmatocystin Treatment

作　　者：张祥宏[1] 谢同欣[1] 严霞[1] 左连富[1] 王凤荣[1] 

机构地区：[1]河北医学院基础所病理室

出　　处：《中华物理医学杂志》1995年第2期80-83,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金

摘　　要：以人胚肺组织体外培养和流式细胞光度术相结合的方法，分析河北省肿瘤高发区饮食优势污染霉菌毒素－杂色曲霉素处理后的人胚肺组织细胞ＤＮＡ含量、增殖状态和抑癌基因ｐ５３、癌基因ｒａｓｐ２１蛋白表达的变化及其相互关系．结果表明，杂色曲霉素处理后，体外培养的人胚肺细胞周期Ｓ期细胞比例、增殖指数明显增高，ｐ５３和ｒａｓｐ２１均出现过度表达，提示杂色曲霉素可诱发人胚肺细胞恶性转化。Sterigmatocystin(ST)is one of the predominant contamination mycotoxins in foodstuff of high risk area of cancer in Hebei province.The effects of ST treatment on DNA contents,cell proliferation,p53 gene and oncogene ras p21 expression in human fetal lung cells in vitro were studied with immuno-flow cytometry.The results revealed that after ST treatment.The human fetal lung cell showed dramatical increases in S phase fraction and cellular proliferation indice,p53 and ras p21 protein products overexpression.All these suggest that ST could induce malignant transformation in human fetal lung cells in vitro.

关 键 词：杂色曲霉素 P53 RASP21 转化 肺肿瘤 癌基因 

分 类 号：R734.202[医药卫生—肿瘤]