维生素B＿2产生菌的原生质体制备及再生  

Preparation and Regeneration of Protoplasts from Vitamin B_2 Producer Eremothecium aohbyii

作　　者：王俊刚[1] 朱宝成 安海谦[1] 李庆余 

机构地区：[1]承德医学院生物教研室

出　　处：《河北大学学报（自然科学版）》1995年第1期75-77,共3页Journal of Hebei University(Natural Science Edition)

摘　　要：用１．０％纤维素酶＋０．５％蜗牛酶的混合酶液酶解培养２６～３２小时的维生素Ｂ＿２产生菌阿氏假囊酵母（Ｅｒｅｍｏｔｈｅｃｉｕｍａｏｈｂｙｉｉ）菌丝体，可以得到大量原生质体，最佳酶解时间为１．５小时。原生质体再生率为０．３５％～０．６７％。阿氏假囊酵母在原生质体再生过程中具有较高的突变率，以维生素Ｂ＿２产量为指标的正突变率为１２．１４％。Using a combined enzyme system containing 1.0% cellulase(ONOZUKAR-10)plus 0.5 % snail digestase,high yield of protoplasts from Eremothecium aohbyiimycelia which had grown for 26-32 hours was obtained.The regeneration frequency were0.35%-0.67%. Many mutants appeared in the regeneration strains on solid medium. Posi-tive mutation ratio was 12.14% by determining the yield of Vitamin B_2.

关 键 词：阿氏假囊酵母 原生质体 再生 维生素B2 发酵 

分 类 号：TQ924[轻工技术与工程—发酵工程] TQ466.2