苯丙氨酸的脱氨酶法测定  

Determination of Phenylalanine by Deaminase

作　　者：范长胜[1] 胡宝龙[1] 杨仁根 

机构地区：[1]复旦大学微生物学系

出　　处：《氨基酸和生物资源》1995年第3期33-35,共3页Amino Acids & Biotic Resources

摘　　要：方法的原理是：由某些微生物所产生的苯丙氨酸脱氨酶能使苯丙氨酸分子氧化脱氨，生成相应的苯丙酮酸。在合适的反应体系中，苯丙酮酸与三氯化铁反应生成的化合物呈蓝绿色，这种颜色的深浅与苯丙氨酸含量成正比，制备的标准曲线有良好的线性。This method is used for determination of phenylalanine in solution.It is based on that phenylalanine-deaminase, extracted from microbes, catalyzes the deamination of L-phenylalanine to produce phenylpyruvate. Then the phenyl-pyruvate reacts with ferric chloride to form a compound of blue-green colour. In suitable reaction system, various shades of the colour direactly proportional are sdirectly proportional to the concentrations of phenylalanine.

关 键 词：苯丙氨酸 氨基酸 脱氨酶法 分析 

分 类 号：Q517.03[生物学—生物化学]