男性胃癌病人Y染色体缺失的原位杂交检测及其意义  

THE USE OF IN SITU HYBRIDIZATION TO DETECT THE LOSS OF Y CHROMOSOME IN MALES WITH GASTRIC CARCINOMA

作　　者：田新霞[1] 雷道年[1] 张平[1] 王申五[1] 朱玲[1] 林三仁[1] 

机构地区：[1]北京医科大学病理学系,北京医科大学血液病研究所,北京医科大学第三医院消化科

出　　处：《北京医科大学学报》1995年第2期87-89,共3页Journal of Peking University(Health Sciences)

基　　金：国家"八五"攻关课题。

摘　　要：建立了用染色体特异的探针检测细胞染色体畸变的原位杂交法。用生物素标记的ＰＹ３．４探针对１２例男性胃癌标本进行原位杂交，检测Ｙ染色体的丢失，我们发现：胃癌组织细胞的Ｙ染色体丢失率很高，Ｙ染色体丢失很可能是胃癌细胞的一个染色体标志，胃癌组织细胞的Ｙ染色体丢失与肿瘤的分化程度和肿瘤的转移有一定关系，这对胃癌的诊断和估计预后有一定帮助。his report investingated the use of in situ hybridization for detection of chromtosome aberrations with chro- mosome-specific DNA probes. Fluorescent in situ hybridization(FISH) with biotinylated PY3.4 probe was used for the detection of loss of the Y chromosome of twelve male gastric carcinomas,.We found that the loss of Y chromosome from the gastric carcinoma was very significant, it possibly represented a chromosomal marker in this type of cancer and would be useful to diagnosis of gastric carcinoma. We also found that the loss of Y chromosome from the gastric carcinoma correlated with the degree of differentiation and the metastasis of tumors,which would help estimate prognosis of tumor.

关 键 词：胃肿瘤/遗传学 染色体缺失 原位杂交 

分 类 号：R735.202[医药卫生—肿瘤]