c－Ha－ras反义DNA及其衍生物对转化细胞恶性表型的逆转  

REVERSION OF MALGNANT PHENOTYPE OF THE TRANSFORMED CELLS BY ANTSENSE C-Ha-Ras DNA AND ITS PSORALEN DERIVATIVE

作　　者：杨定成[1] 战洪生[1] 张礼和[1] 李通[1] 藏传兵 王来新 吕有勇[1] 

机构地区：[1]北京医科第一医院外科,北京医科大学天然及仿生药物国家重点实验室,北京市肿瘤防治研究所生化室

出　　处：《北京医科大学学报》1995年第5期353-355,369,共4页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金及国家重点实验室资助课题

摘　　要：应用突变ｃ－Ｈａ－ｒａｓ质粒ＤＮＡ转化小鼠Ｃ３Ｈ１０Ｔ１／２纤维细胞，发现细胞获得恶性表型。本文合成了反义ＤＮＡ（ｏｌｉｇｏ），它与ｃ－Ｈａ－ｒａｓ基因前４个密码子及其上游正链序列互补；并发现ｏｌｉｇｏ可有效地抑制恶性转化细胞内ｃ－Ｈａ－ｒａｓ基因表达、细胞在软琼脂中集落形成以及细胞在裸鼠体内的致瘤作用。补骨脂素修饰ｏｌｉｇｏ后，其生物学活性增强。n this study ,the mice fibroblast cell line-C3H10T1/2 was transformed with mutated c-Ha-ras plasmid DNA.The transformed C3H10T1/2 cell obtained the malignant phenotype. The antisense c-Ha-ras DNA (oligo) was syn-thesized in this study. The oligo was complementary to the single positive strand of the first four codons and the upstream sequence of c-Ha-ras gene.We found that the expression of activated c-Ha-ras oncogene,foci formation in soft agar and tumorigenicity in nude mice of the transformed C3H10T1/2 cells could be effectively inhibited by oli- go.When psoralen group was covalently linked to the 5’-end of the oligo,the biological effect of oligo was en-banced.

关 键 词：胃肿瘤 原癌基因蛋白 反义寡核苷酸 基因表达 

分 类 号：R735.202[医药卫生—肿瘤]