海带基因工程选择标记的研究  

STUDIES OF SELECTIVE MARKER FOR KELP TRANSFORMATION

作　　者：武建秋[1] 王希华[1] 秦松[1] 曾呈奎[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》1995年第5期42-45,共4页Marine Sciences

摘　　要：海带幼孢子体对抗生素的敏感性实验证明，氯霉素可以作为海带基因工 程中较理想的选择压力。本文得出了氯霉素对海带的半致死剂量，提示 ＣＡＴ（氯霉 素乙酸转移酶）基因可以用作海带基因工程的阳性选择标记基因。In recent years, progress has been made in the gene transfer of seaweeds. Lack of selective marker made it difficult to acquire a stoblyexpressed transgenic kelp. Results of antibiotic sensitivity of young sporophytes of Laminaria japonica was reported in the present paper. It is proved that, Laminaria japonica is very sensitive to chloromycetin. LD60h50of young sporophytes (1. 5cm-2. 0cm in length) to of chloromycetin is 188. 8ug/ml and LD72h50is 154. 6ug/ml. LD60h50 of sporophytes from 3. 5cm to 4. 0cm in length is 406. 5ug/ ml and LD72h50 is 184. 6ug/ml. These results indicate that chloromycetin can be a selective marker and CAT gene will be a promising positive seloctive gene for kepl transformation.

关 键 词：海带 基因工程 选择标记 抗生素 氯霉素 

分 类 号：Q949.22[生物学—植物学]