单克隆抗体酶免疫分析法测定大米中杀虫脒残留量  

Determination of Chlordimeform Residues in Rice by Enzyme Immunoassay Using Monoclonal Antibody

作　　者：余万俊[1] 鲁长豪[1] 肖志芳[1] 牟家婉 蒋中华 

机构地区：[1]华西医科大学公共卫生学院

出　　处：《环境科学》1995年第4期51-53,共3页Environmental Science

基　　金：国家教委博士点基金;CMB基金

摘　　要：用抗杀虫脒单克隆抗体为试剂，建立了间接竞争法、直接竞争法和标记抗原的竞争法等３种测定大米中杀虫眯残留量的ＥＬＩＳＡ法，标准曲线上抑制率为５０％时对应的杀虫眯浓度分别为１．３、１．７和５．３ｎｇ／ｍｌ。大米样品用甲醇提取，提取液经吸附剂柱简单净化后即可用上述ＥＬＩＳＡ对痕量杀虫眯进行定量。使用直接竞争法时，样品加标回收率为９０％－１１６％，平行测定的相对标准偏差为５．ｌ％－１６％，方法检出限为０．５ｎｇ／ｇ。笔者还对孵育介质ｐＨ和温度、提取剂及样品基体等因素对定量曲线的影响进行了研究和讨论。Three sensitive enzyme-linked immunosorbent assays(ELISAs) indirect competitive ELISA,direct competitive ELISA, and reverse direct competitive ELISA-with immobilized antibody were developed for the determination of chlordimeform residues in rice. Their IC50 are of 1. 3, 1. 7 and 5. 3 ng/ml, respectively. Samples were homogenized with methanol in a blender or in an ultrasonic disintegrator. The extracts could be measured directly after dilution, or underwent a simple clean up procedure, which could improve the accuracy of the ELISAs. Effects of PH in the reaction medium, the incubation temperature,the extracting agent and the sample matrix on the detection sensitivities were also studied and discussed.

关 键 词：杀虫脒 农药 残留量 免疫分析法 大米 

分 类 号：TS207.53[轻工技术与工程—食品科学]