转 Bt 基因玉米对粘虫的室内杀虫效果评价  

Evaluation the control effects of the transgenic Bacillus thuringiensis corn expressing CrylAb protein on the larvae of Mythimna separata (Walker) in laboratory

作　　者：王振营[1] 王冬妍[1,2] 何康来[3] 白树雄[3] 丛斌[1] 

机构地区：[1]中国农业科学院植物保护研究所 [2]沈阳农业大学植物保护学院,沈阳110161 [3]沈阳农业大学植物保护学院

出　　处：《植物保护学报》2005年第2期153-157,共5页Journal of Plant Protection

基　　金：国家"863"课题(2002AA212161)资助

摘　　要：在室内用生测法研究了表达 CrylAb 杀虫蛋白的 Bt 玉米 MON810和 Btll 小苗和心叶对粘虫 Mythimna separata (Walker)初孵幼虫以及玉米叶片对暴食期4龄和5龄幼虫的杀虫效果。结果表明,2种 Bt 玉米小苗及心叶对粘虫初孵幼虫都具有很好的控制效果,取食 MON810和 Btll 小苗的粘虫初孵幼虫第1天的存活率分别为69.44％和86.11％,到第3天时,取食 Btll 小苗的幼虫全部死亡,此时取食 MON810小苗的幼虫存活率仅为4.17％,到第7天全部死亡,而此时在各自相对应的对照玉米 DK647和 NX4906小苗上幼虫存活率分别为91.66％和88.89％。取食 MON810和Btll 心叶的粘虫初孵幼虫第2天时幼虫存活率急剧下降,分别为13.89％和19.45％,到第7天时,取食两种 Bt 玉米心叶的初孵幼虫全部死亡,而取食对照玉米心叶幼虫存活率分别为94.45％和97.22％,差异极显著。两种 Bt 玉米对粘虫4龄幼虫有很好的杀虫效果,取食的第11天4龄幼虫全部死亡,对照幼虫存活率均为100％;在 Btll 和 MON810叶片上取食的粘虫5龄幼虫也不能存活,分别在取食的第11天和12天全部死亡,对照存活率分别为96.00％和98.00％,差异极显著。表明 Bt 玉米对粘虫初孵幼虫和暴食期的4龄和5龄粘虫都具有显著的致死作用。Control effects of the seedlings, whorl leaves and the leaves of transgenic Bacillus thuringiensis corn on the 1 st instar, 4th instar and 5th instar larvae of Mythimna separata （Walker） were evaluated in laboratory. Two Bt corn hybrids of events MON810 and Bt11 expressing Cry1Ab protein and their non-Bt controls were employed in the bioassays. Results showed the survival was 4.17% and 0 at the 3rd day when the 1st instar larvae feeding on the seedlings of MON810 and Bt11, compared with 91.66% and 88.99% in corresponding non-transgenic isogenic hybrid, respectively. The survival rate was 13.89% and 19.45% for the 1st instar larvae when feeding on whorl leaves of MON810 and Btl 1 at the 2nd day, respectively, and none survived at the 7th day. The mortality was 100% for 4th instar larvae of M. separate when feeding on the leaves of MON810 and Bt11 at the 11th day, compared with 0 mortality in non-transgenic isogenic hybrid. The 4th and 5th instar larvae exhibited weight loss when fed on MON810 and Bt11 leaves, and showed weight gain when fed on two non-Bt controls.It was shown that the Bt corn expressing Cry1 Ab toxin had good control effects on M. separate, not only the 1 st instar larvae, but also the 4th and 5th instar larvae.

关 键 词：转BT基因玉米 粘虫 杀虫效果 CrylAb杀虫蛋白 幼虫 室内生物测定法 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]