多发性骨髓瘤N-Ras pERK1/2的表达及相互关系的探讨  

Investigation on Relationship and Over-expression between Non-mutated N-Ras and Signal Protein pERK1/2 in Human Multiple Myeloma

作　　者：樊华[1] 金锋[2] 张国君[1] 王萍萍[1] 王艳萍[1] 卢香兰[1] 李霞[1] 

机构地区：[1]中国医科大学附属第一医院血液科,沈阳市110001 [2]中国医科大学附属第一医院肿瘤科,沈阳市110001

出　　处：《中国肿瘤临床》2005年第14期792-794,共3页Chinese Journal of Clinical Oncology

基　　金：留学回国人员科研基金资助(编号:2000367)

摘　　要：目的:观察N-Ras/pERK1/2在多发性骨髓瘤(MultipleMyeloma,MM)中的表达及临床意义,探讨N-Ras/MAPK信号通路与多发性骨髓瘤发生、发展之间的相互关系。方法:选择多发性骨髓瘤患者28例,应用Westernblot印记杂交检测N-Ras、pERK1/2蛋白表达水平,分析两者表达的相互关系。应用聚合酶链反应(PCR)/限制性片段长度多肽性分析法(RFLP),单链构象多肽性分析法(SSCP)和DNA测序方法对多发性骨髓瘤患者进行N-Ras基因12位点突变分析。结果:多发性骨髓瘤N-Ras、pERK1/2的表达水平(N-Ras:0.56±0.19;pERK1/2:0.39±0.21)显著高于正常人末梢血淋巴细胞(N-Ras:0.13±0.12;pERK1/2:0.10±0.14()P<0.01);28例多发性骨髓瘤患者中未发现N-Ras基因12位点突变;相关性分析结果表明多发性骨髓瘤N-Ras与pERK1/2的表达具有密切相关性(r=5.1326,P<0.01)。结论:非突变型N-ras基因产物介导的ERK信号传导通路参与了多发性骨髓瘤的发生、发展过程。Objective： To investigate the expression and its clinical significance of mutated/nonmutated （Codon 12） N-Ras and pERK1/2 （Extracellular signal-regulated kinase）, in order to clarify the relationship between expression of Ras and ERK in Multiple Myeloma （MM）. Methods： Western blot analysis was used to detect the expression level of N-Ras and pERK1/2, and mutation analysis at Nras codon 12 was finished using the method of PCR/RFLP, PCR/SSCP and DNA sequencing analysis, in the clinical samples from 28 diagnosed MM patients. Results： The expression level of N-Ras and pERK1/2 （N-Ras. 0.56±0.19; pERK1/2： 0.39±0.21） was significant higher in the clinical samples suffering from multiple myeloma than that in the group of peripheral blood lymphocytes of volunteer （N-Ras： 0.13±0.12; pERK1/2： 0.10±0.14, P〈0.01）. There was no mutation at N-ras codon 12 in all of patients suffering from MM. Close relationship was found between the expression level of non-mutated N-Ras and pERK1/2 in Multiple Myeloma （r=5.1326, P〈0.01）. Conclusions： There is a close relationship between the expression of N-Ras and the pERK1/2 in Multiple Myeloma. Ras/ERK signaling pathway, seldom affected by N-ras （Codon 12） mutation, may play some roles in the development of human Multiple Myeloma.

关 键 词：多发性骨髓瘤 N-RAS ERK 

分 类 号：R733.3[医药卫生—肿瘤]