载脂蛋白E基因多态性与新疆维吾尔族自然长寿的相关性研究  

Polymorphism of apolipoprotein E gene and natural longevity in the Xinjiang Uighur people: an association study

作　　者：玛依拉.吾甫尔 方鸣武[2] 程祖亨[1] 邱长春[2] 

机构地区：[1]新疆医科大学第一附属医院心内科,乌鲁木齐830054 [2]中国医学科学院北京协和医科大学基础医学研究所分子生物学国家重点实验室

出　　处：《中华医学遗传学杂志》2005年第4期462-463,共2页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金(30160089);美国纽约中华医学基金(CMB96657)~~

摘　　要：目的探讨载脂蛋白E(apolipoproteinE,apoE)基因多态性与新疆维吾尔族自然长寿的关系。方法应用聚合酶链反应限制性片段长度多态性方法检测百岁组42名,90岁组102名,65～70岁组70名和对照组53名的apoE基因多态性。结果百岁组apoE的ε3/3、ε2/3和ε3/4基因型频率分别为69.0%、23.8%和2.4%,其ε3、ε2和ε4等位基因频率分别为82.1%、16.7%和1.2%,百岁组ε3/4基因型及ε4、ε3等位基因频率显著低于对照组(P<0.01),ε2/3基因型及ε2等位基因频率则显著高于对照组(P<0.01)。百岁与apoE基因的ε2等位基因呈正关联,与ε4等位基因呈负关联。结论在新疆维吾尔族,apoE基因多态性与个体寿命密切相关,同时也应考虑到长寿是年龄依赖的多种因素影响的结果。Objective To explore the distribution of polymorphisms of apolipoprotein E（apoE） gene and its relation to natural longevity in the Xinjiang Uighur people. Methods Using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）, the authors obtained data from centenarians（ n = 42）, 90-year-old people （ n =102）, 65-70-year-old people （ n = 70） and controls（ n = 53）. Results The frequencies of apoE genotypes ε3/4,ε3/3and ε2/3 were 2.4% ,69.0% and 23.8% respectively in the centenarian group, and the frequencies of their ε4,ε3 and ε2 alleles were 1.2%, 82.1% and 16.7%. The frequencies of genotype ε3/4 and ε4, ε3 allele in the centenarian group were significantly lower than those in controls（ P 〈 0.01 ）, whereas the frequencies of genotype ε2/3 and ε2 allele in the centenarian group were significantly higher than those in controls（ P 〈 0.01 ）. Longevity was noted to be positively correlated with ε2 and negatively correlated with ε4. Conclusion This relationship reflects a genetic influence on differential survival and may point to pleiotmpic age-dependent effects on longevity.

关 键 词：载脂蛋白E 基因多态性 新疆 维吾尔族 寿命 基因型 等位基因 

分 类 号：R394[医药卫生—医学遗传学]