过柱纯化-高效液相色谱法测定玉米黄曲霉毒素  

Determination of aflatoxins in maize by combining high performance liquid chromatography with florisil column.

作　　者：高军侠[1] 倪余文[2] 万昊雷[3] 李桂芳[1] 刘作新[1] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]中国科学院大连化学物理研究所,大连116012 [3]沈阳农业大学生物科技学院,沈阳110161

出　　处：《生态学杂志》2005年第8期967-969,共3页Chinese Journal of Ecology

基　　金：国家粮食丰产科技工程(2004BA520A01-5);中国科学院沈阳应用生态研究所海外学者基金资助项目(SCXLX0204)。

摘　　要：通过硅镁净化柱纯化,利用高效液相色谱法测定了玉米(Zeamays)中的黄曲霉毒素,在短时间(10min)内实现了黄曲霉毒素主要组分B1、G1、B2和G2的分离,确定了它们的回归方程和检测限。并在此基础上,结合目前研究现状,分析了该方法在应用中存在的问题,讨论了B1、G1、B2和G24种主要组分的出峰顺序。该试验结果将有助于高效液相色谱法在检测黄曲霉毒素应用中的日臻完善。Aflatoxins in maize were determined by combining high performance liquid chromatography with florisil column in this paper. The main components, aflatoxin B1, G1, B2 and G2, were separated in short time （10 min）. The regressive equations and detection limits of them were given. Some existed problems were analyzed based present study. Peak sequences were discussed according to the results. It may contribute to make the method perfected in the practice.

关 键 词：黄曲霉毒素 高效液相色谱法 出峰顺序 

分 类 号：TS207[轻工技术与工程—食品科学]