中国人Bat26多态性及其在胃癌微卫星不稳定性研究中的意义  

Germline polymorphisms of Bat26 and its significance in gastric cancer microsatellite instability

作　　者：吕炳建[1] 来茂德[2] 黄琼[2] 盛宏强[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院病理科,浙江杭州310016 [2]浙江大学医学院病理学与病理生理学系,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2005年第4期353-357,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省科技厅重点科研基金项目(011107607)

摘　　要：目的:探讨中国人Bat26多态性及其在胃癌微卫星不稳定性(MSI)研究中的应用价值。方法:运用PCR-变性聚丙烯酰胺凝胶电泳-银染方法检测389份外周血Bat26,34例胃癌组织和正常黏膜还检测Bat26和其它11个微卫星位点。结果:①423份中国人外周血或正常黏膜Bat26凝胶电泳未发现条带改变。②34例胃癌发现2例MSI-H,均为Bat26+,Bat26改变和MSI-H有很好的一致性(P<0.05)。③与RER-胃癌比较,MSI-H(RER+)胃癌上皮内淋巴细胞、瘤周淋巴细胞浸润更明显,更易表现为推进性生长(P<0.05)。结论:①国人基因组Bat26为近单态性,可不设正常对照用于肿瘤MSI研究。②Bat26改变反映胃癌的MSI-H状态,且MSI-H胃癌有明显的临床病理特点。Objective： To detect the germline polymorphic variations of Bat26 in Chinese and its significance in microsatellite instability （MSI） study of gastric cancers. Methods： Bat26 was analyzed by PCR-based denatured polyacrymide gel electrophoresis-silver stain method in peripheral blood from 389 healthy people and 34 gastric cancers with matched normal mucosa. Eleven other microsatellite loci were also detected for gastric cancers. Results： （1）No Bat26 variations were identified in 423 genomic DNA from peripheral blood or normal mucosa by polyacrymide gel electrophoresis. （2）Two MSI-H cancers, both Bat26 ＋, were detected in 34 cases of gastric cancer. The alterations of Bat26 and MSI-H status were identical （P〈0.05）. （3） Compared with those of RER ancers, MSI-H （RER ＋ ）cancers showed more obvious infiltration of intraepithelial lymphocytes and peri-tumoral lymphocytes,and more pushing borders （P 〈 0. 05）. Conclusions.. （1） The germline polymorphisms of Bat26 in Chinese people are quasimonomorphic. Thus, no matched genomic DNA is needed while Bat26 was selected for tumor MSI analysis. （2）Bat26 is an independent indicator of MSI-H gastric cancers with distinct clinicopathological features.

关 键 词：胃肿瘤/病理学 微卫星不稳定性 Bat26 多态性 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]