Effects of navelbine and docetaxel on gene expression in lung cancer cell strains  

作　　者：Li CAI Hai-ying DONG Guang-jie SUI 

机构地区：[1]Internal Medicine of Oncology, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China

出　　处：《Acta Pharmacologica Sinica》2005年第8期1017-1024,共8页中国药理学报（英文版）

基　　金：a Repatriation Grant from the Foundation of Science Bureau of Harbin, China (2003AFLXJ009);Overseas Scholar Grant from the Department of Education of HeiLongJiang Province,China(1055HZ020)

摘　　要：Aim： To search genes sensitivity to the anti-cancer drugs navelbine （NVB） and docetaxel （DOC） in small-cell lung cancer （SCLC） and non-small-cell lung cancer（NSCLC） cell strains. Methods： The sensitivity of 4 strains of SCLC and 6 strains of NSCLC to NVB and DOC was evaluated using the MTT assay. The expression of 1291 sensitive-related genes to the anti-cancer drugs in 10 lung cancer cell strains was measured using cDNA macroarrays and the relationship was analyzed.Results： In total, there were 56 （r≥0.4） genes sensitive to NVB and DOC. For NVB： 36 genes were sensitive to NVB, 20 co-expressed genes between the SCLC＋NSCLC set and the NSCLC set; 27 expressed genes and 7 specially expressed genes in the SCLC＋NSCLC set; and 29 expressed genes and 9 specially expressed genes in the NSCLC set. For DOC, 50 genes were sensitive to DOC, 12 co-expressed genes between the SCLC＋NSCLC set and the NSCLC set; 24 expressed genes and 12 specially expressed genes in the SCLC＋NSCLC set; and 38 expressed genes and 26 specially expressed genes in the NSCLC set. The genes sensitive to NVB and DOC in lung-cancer cell stains were mainly divided into the following 4 categories： signal transduction molecules, cell factors, transcription factors and metabolism-related enzymes and inhibitors. Conclusions：There were obvious differences in genes related to NVB and DOC between SCLC and NSCLC cell strains, but the same as categories of function.Aim： To search genes sensitivity to the anti-cancer drugs navelbine （NVB） and docetaxel （DOC） in small-cell lung cancer （SCLC） and non-small-cell lung cancer（NSCLC） cell strains. Methods： The sensitivity of 4 strains of SCLC and 6 strains of NSCLC to NVB and DOC was evaluated using the MTT assay. The expression of 1291 sensitive-related genes to the anti-cancer drugs in 10 lung cancer cell strains was measured using cDNA macroarrays and the relationship was analyzed.Results： In total, there were 56 （r≥0.4） genes sensitive to NVB and DOC. For NVB： 36 genes were sensitive to NVB, 20 co-expressed genes between the SCLC＋NSCLC set and the NSCLC set; 27 expressed genes and 7 specially expressed genes in the SCLC＋NSCLC set; and 29 expressed genes and 9 specially expressed genes in the NSCLC set. For DOC, 50 genes were sensitive to DOC, 12 co-expressed genes between the SCLC＋NSCLC set and the NSCLC set; 24 expressed genes and 12 specially expressed genes in the SCLC＋NSCLC set; and 38 expressed genes and 26 specially expressed genes in the NSCLC set. The genes sensitive to NVB and DOC in lung-cancer cell stains were mainly divided into the following 4 categories： signal transduction molecules, cell factors, transcription factors and metabolism-related enzymes and inhibitors. Conclusions：There were obvious differences in genes related to NVB and DOC between SCLC and NSCLC cell strains, but the same as categories of function.

关 键 词：cDNA macroarray lung neoplasms NAVELBINE DOCETAXEL 

分 类 号：R734.2[医药卫生—肿瘤]