辣椒素诱导人黑色素瘤A375-S2细胞凋亡的研究  

Mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells

作　　者：龚显峰[1] 王敏伟[1] 池岛乔[1] 

机构地区：[1]沈阳药科大学中日医药研究所,110016

出　　处：《中华肿瘤杂志》2005年第7期401-403,共3页Chinese Journal of Oncology

摘　　要：目的研究辣椒素对人黑色素瘤A375S2细胞凋亡的诱导作用,探讨其抗癌作用机制。方法采用噻唑蓝(MTT)法观察辣椒素对细胞生长的抑制作用,荧光显微镜下观察细胞的形态学变化,通过DNA凝胶电泳进行DNA片段化分析,流式细胞术检测细胞凋亡,Westernblot法分析DNA酶抑制剂(ICAD)蛋白的表达。结果辣椒素可诱导A375S2细胞凋亡,呈剂量、时间依赖性。250μmol/L辣椒素处理24h,A375S2细胞出现明显的亚二倍体峰;处理36h,Hoechst33258荧光染色有明显的凋亡小体出现,DNA电泳出现阶梯状图谱。在辣椒素作用下,caspase激活的ICAD蛋白表达量随时间延长而减少。结论辣椒素对A375S2细胞的杀伤作用主要通过诱导细胞凋亡实现,降低ICAD蛋白的表达为其诱发A375S2细胞凋亡的机制之一。Objective To study the mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells. Methods MTF assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment. Results Capsaicin induced A375-S2 cell death in a time- and dose-dependent manner.Sub-diploid peak was seen at 24 h ＇after 250 μmol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase（ ICAD）was reduced with the lapse of time. Conclusion Capsaicin induces A375-S2 cell apoptosis and down-regulation of ICAD contributes to this process.

关 键 词：辣椒素 诱导 黑色素瘤 A375-S2细胞 细胞凋亡 

分 类 号：R739.5[医药卫生—肿瘤]