纤维素酶活力测定条件研究  

Studies on Condition for Determination of Cellulase Activity

作　　者：夏服宝[1] 邱雁临[1] 孙宪迅[1] 

机构地区：[1]湖北工业大学生物工程学院

出　　处：《饲料工业》2005年第16期23-26,共4页Feed Industry

摘　　要：用DNS法测定纤维素酶活力,分析测定条件对测定结果的影响。在不同的条件下测定酶活力会得到不同的纤维素酶活力测定值。其中,酶液用量、酶解反应时间、温度对测定结果影响较大,酶液的稀释度、DNS显色时间对测定结果也有一定影响。测定纤维素酶活力的最适条件为:酶解反应时间为30min,最适温度40℃,DNS用量2.5ml,显色时间5min。The purpose of this study is analyzing the effects of determination conditions on results of measurements of by the DNS method. On different conditions, the results ot the measurements ot cellulase activity are different. Among these conditions, the concentration of eellulase, reactions time and temperature have great effects on the results. The optimum conditions for determination of cellulase aetivity： 30min for enzymatic reaction; optimum temperature 40℃, the quantity of DNS is 2.5ml and 5min for cloring.

关 键 词：纤维素酶 酶活力 测定条件 

分 类 号：S816.17[农业科学—饲料科学]