抑制差减杂交筛选辐射致癌差异表达基因  

Study on radiation carcinogenesis-related differentially expressive genes with suppressive subtractive hybridization

作　　者：熊杰[1] 韩玲[1] 朱丹[1] 高建国[1] 

机构地区：[1]第二军医大学海军医学系放射医学教研室,上海200433

出　　处：《中华放射医学与防护杂志》2005年第4期322-324,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(30170282)

摘　　要：目的筛选研究辐射诱发小鼠肿瘤相关差异表达基因。方法以60Coγ射线照射诱发的小鼠白血病动物模型为研究对象,采用抑制差减杂交技术构建辐射致癌差异表达基因cDNA文库,鉴定后挑取阳性克隆测序,并与GeneBank数据库进行同源性比对分析。结果抑制差减杂交筛选得到102个阳性克隆,随机挑选30个克隆进行菌液PCR,扩增得到28个特异性插入片段,DNA测序结果经与GeneBank数据库比对发现代表了24个已知基因,其中3个为重复检出基因,暂未发现有未知序列基因。结论成功建立辐射致癌差异表达基因cDNA文库,初步筛选获得24条表达上调的差异片段,该结果将有助于充实完善辐射致癌的分子机制。Objective To research differentially expressive genes related with radiation induced tumors in mice. Methods A mouse leukemia model was induced by ^6o Co γ-ray irradiation and the cDNA library of differentially expressive genes between normal thymus and thymus tumor was established with suppressive subtractive hybridization. Positive clones were identified and studied respectively by DNA sequencing, Results A total of 102 positive clones were screened out by suppressive subtractive hybridization. After PCR for 30 randomly selected clones, 28 clones showed anticipated inserted cDNA sequence, These cDNA sequences represented 24 known genes including three repeatedly detected genes comparing with GeneBank database, Conclusions The cDNA library of differentially expressive genes related to radiation carcinogenesis was successfully established and 24 ascendingly expressive genes were screened out, This study will contribute to enrich the molecular mechanism of radiation carcinogenesis.

关 键 词：差减杂交 筛选技术 辐射诱导 癌差异表达基因 小鼠 ^60Coγ射线 白血病 

分 类 号：R730.23[医药卫生—肿瘤]