微孔草油中脂肪酸的分离和鉴定  

ISOLATION AND IDENTIFICATION OF FATTY ACID COMPONENT FROM SEED OIL OF MICROULA SIKKIMENSIS(CLARKE)HEMSL

作　　者：李京民[1] 王静萍[1] 于凤兰[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《Acta Botanica Sinica》1989年第1期50-53,共4页Acta Botanica Sinica（植物学报：英文版）

摘　　要：微孔草[Microula sikkimensis(Clarke)Hemsl.]种子含油45%,其油中含有γ-亚麻酸,(顺式-6,9,12-十八碳三烯酸)8.1%。通过硝酸银硅胶柱层析和高压液相色谱分离,采用高碘酸钠、高锰酸钾氧化裂解的方法和红外光谱,紫外光谱,气相色谱分析,质谱分析,对油中的γ-亚麻酸,顺式-6,9,12,15-十八碳四烯酸,顺式-11-廿碳烯酸,顺式-13-廿二碳烯酸,顺式-15-廿四碳烯酸进行了分离、鉴定。The seed oil content of Microula sikkimensis(Clarke)Hemsl.is up to 45%.There is 8.1% of γ-linolenic acid which has the pharmacological action in the fatty acids composition.It has showed that this oil has a stronger effect on reducing triglyceride in serum.Fifteen diffe- rent kinds of fatty acids were analysed.The unsaturated C_(20),C_(22),C_(24),acid,C_(18)triene-acid and tetraene-acid of the seed oil were separated on AgNO_3-silica gel column and HPLC.and were identified by Periodata-Permanganate Oxidation,GLC,IR,UV,and MS.They are cis- 11-eicosenoic,cis-13-docosenoic,cis-15-tetracosenoic,cis-6,9,12-octadecatrienoic and cis-6,9,12,15— octadecatetraenoic acids.

关 键 词：微孔草 种子油 脂肪酸 Γ-亚麻酸 

分 类 号：S565.9[农业科学—作物学]