人精子染色体制备方法的改良与应用  

Study on Preparation and Clinical Application of Human Sperm Chromosome

作　　者：王世雄[1] 黄荣魁[1] 胡琴 李一峰 曹英 

机构地区：[1]上海第二医科大学,上海市儿科医学研究所

出　　处：《上海第二医科大学学报》1995年第3期235-238,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：对８０例正常人组进行精子染色体直接制备、Ｇ显带核型分析，成功率为６２．５％，与Ｔｅｍｐｌａｄｏ方法５８．１％比较，Ｐ＜０．０５，有差异。精子染色体数目和结构畸变率分别为２．３％和０％。另对４６例临床对象进行精子染色体研究，精子染色体数目和结构畸变率分别为：不育组１５例，１４．０％和４．８％；流产组２６例，４．５％和２．４％，均较正常人组增加。染色体平衡易位携带者组５例，均发现中期Ⅰ易位型染色体的环状四价体核型。In this study,the direct preparation and G-banding karyotype of human sperm chromosome were carried out in 80 male persons asnormal controls. The success rate was discovered to be 62.5%, which was higher than that obtained from the method previously reported by Templado (58.1%, P<<.05). The same chromosomal analysis of human sperm was simultaneously performed in 46 clinical cases, including 15 in infertility group, 26 in abortion group and 5 in group of balanced chromosomal translocation. As a result the appearance rates of numerical and/or structural aberrations were found in control group (2.3%, 0%),infertility group (14.0% 4.8%), and abortion group (4.5%, 2.4%), respectively. It was shown that the abnormal rate in infertility and abortion groups was evidently higher than that in normal control. Moreover, to ring quadrivalents were identified in 5 carriers with balanced chromosomal translocation.

关 键 词：精子 制备技术 G显带核型 染色体显带 

分 类 号：R697[医药卫生—泌尿科学] Q343.2[医药卫生—外科学]