人精子染色体改良技术及其应用  

THE IMPROVED TECHNIQUES AND APPLICATIONS OF HUMAN SPERM CHROMOSOME

作　　者：王世雄[1] 黄荣魁[1] 胡琴[1] 李一峰[1] 曹英[1] 

机构地区：[1]上海市儿科医学研究所,上海第二医科大学附属新华医院

出　　处：《上海医学》1995年第1期1-4,共4页Shanghai Medical Journal

基　　金：上海市科委资助

摘　　要：课题研究提供了改良的人精子染色体直接制备、Ｇ显带核型分析技术。在对８０例对象的人精子染色体直接制备、Ｇ显带核型分析中，成功率为６２．５％，较Ｔｅｍｐｌａｄｏ方法的成功率（５８．１％）进一步提高。通过对正常人、不育、流产对象的男性精子染色体研究，发现精子染色体数目和结构畸变率分别为：正常人２．３％和０％，不育组１４．０％和４．８％，流产组４．５％和２．４％，不育和流产组的畸变率较正常人增加。在对５例染色体平衡易位携带者的精子染色体分析中，均发现中期Ⅰ易位型染色体的环状四价体核型。The improved techniques of human sperm chromosome were provided and some clini-cal practice were used on the study.80 cases were studied with direct chromosomalpreparation and G- banding karyotype.The successful rate was 62.5%,Which was higherthan the methods reported by Templado,et al.The clinical study was performed innormal controls and cases of infertility,spontaneous abortion and balanced translocation. The rates of numerical and structural chromosomal aberrations were found in normalcontrols(2.3%,0%),infertile group(14 .0%,4.8%)and abortive group(4.5%,2.4%),respectively. The ring quadrivalents were identified in 5 carriers with balancedchromosomal translocation.

关 键 词：人 精子 染色体 改良技术 制备 

分 类 号：R698.204[医药卫生—泌尿科学]