用中性红标记酵母原生质体初探  

Preliminary Research on Marking Yeast Protoplast with Netural Red

作　　者：吴根福[1] 沈煜[1] 应伟军 

机构地区：[1]杭州大学生物科学与技术系

出　　处：《生物技术》1995年第6期23-25,12,共4页Biotechnology

摘　　要：用２％蜗牛酶处理酵母细胞６０分钟，啤酒酵母Ｙ２９的原生质体形成率为９０％，再生率为９．５％；糖化酵母ＩＢ的原生质体形成率为８６％，再生率为１２％。用５００ｐｐｍ中性红染液对Ｙ２９菌株的整细胞和原生质体染色１５分钟，细胞的着色率为８４％，存活率为１２％，而原生质体的着色率为７５％，再生率为６．４％．经染色后的原生质体体积缩小，在突变电场中排队所需的场强也降低。The yeast protoplasts were made with common method, and they were detained with neturared. The results showed that after treating yeast cells with 2% snail enzyme for 60 min.Protoplast formation and regeneratioll frequency were 90% and 9. 554 respectively to S.cerevisiae Y29; 86% and 12% respectively to S. diastatics IB. After detaining Y29 cells andprotoplasts with 500ppm netural red for 15 min. Colored cell rate was 84%, survuved cell ratewas 12% ; Colored protoplast rate was 75%, regeneration rate was 6. 4%. The results alsoshowed that detaining can make cells shrinked, the line-up volatage lowed. In order to makefusion successful, authors ssuggested detained diploid or polyploid protoplasts fuse withundetained haploid protoplasts.

关 键 词：酵母 原生质体 中性红 标记 

分 类 号：Q939.504[生物学—微生物学] Q78