快速检测猪肉中单核细胞增多症李氏菌PCR试剂盒的研制  

THE DEVELOPMENT AND APPLICATIONOF PARID PCR KIT FOR DETECTION OF LISTERIA MONOCYTOGENES IN PORK

作　　者：杨百亮[1] 丁伟[1] 薛应照 

机构地区：[1]山西农业大学动医系,山西临猗县动检站

出　　处：《微生物学通报》1995年第3期160-163,共4页Microbiology China

基　　金：山西省青年科学基金和省计委资助项目

摘　　要：快速检测猪肉中单核细胞增多症李氏菌的ＰＣＲ试剂盒与分离培养法平行检测１５６份猪肉样品，结果ＰＣＲ的阳性率为５．８％，分离培养法的阳性率为３．８％，两者符合率为９７％。实验证明，该试剂盒具有良好的特异性和可重复性，可测出样品中至少３２ＯＣＦＵ细菌，并在２ｄ内取得结果，具有推广应用价值。A kit of rapid PCR for detection of L, monocytogenes has been developed and used todetectthe bacteria in pork samples in paralell with the routine culture method with the result that positive ratewas 5.8%for the former and 3.8% for the latter,and as few as 320 CFU bacteria could be detected。No significant differences were found between the results of both methods(p >0. 05).The results alsoshowed that the rapid PCR kit had good specificity and reproductivity,and required less than 48h to gofrom sample to a final result.It is concluded that the rapid PCR kit is applicable to detect L。monocytogenes in pork。

关 键 词：PCR试剂盒 单核细胞增多症 李氏菌 猪肉 检测 

分 类 号：R392－33[医药卫生—免疫学] TS207.4[医药卫生—基础医学]