一株高效脱酚菌麦芽糖假丝酵母10－4的研究  

STUDY ON ONE STRAIN OF HIGH EFFECTIVE DEGRADING PHENOL MICROBE CANDIDA MALTOSA

作　　者：杨彦希[1] 尹萍[1] 杨惠芳[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学通报》1995年第4期208-211,共4页Microbiology China

基　　金：国家自然科学基金

摘　　要：从炼油厂严重污染的污水和土壤中分离筛选到１２株假丝酵母及丝孢酵母，能以苯酚为唯一碳源生长，２８．４０ｈ内降解苯酚可达１２００ｍｇ／Ｌ以上。其中１０－４号菌株降解苯酚浓度最高可达１７００ｍｇ／Ｌ。在含５００ｍｇ／ｌ苯酚及２０ｍｇ／ＬＣＮ－（５０ｍｇ／ＬＫＣＮ）的培养液中，降解苯酚的同时能降解１３．７ｍｇ／Ｌ的ＣＮ￣－。鉴定为麦芽糖假丝酵母（Ｃｏｎｄｉｄａｍａｌｔｏｓａ）。其生长的最适ｐＨ范围为６－９，降解苯酚的最适ｐＨ范围为４－９，生长的最适温度为２５－３２℃，降解苯酚的最适温度为２８－３７℃。生长与降解苯酚均需通气条件。加入氮源能促进生长和苯酚降解。Twelve strains of yeast belonging to Candida and Trichosporon have beenlsolated from sewage and soil,which polluted by oil and its constituents from oil refinery.These yeasts can use phenol as sole carbon source for growth and degrade phenol more than1200 mg/L in 28-40 hours. Among them strain 10-4 is the most effective one. It is ableto degrade 1700 mg/L of phenol.Moreover it can degrade not only total phenol but also13.7 mg/L of CN in medium containing 500 mg/L of phenol and 20 mg/L of CN￣- .It hasbeen identified as Candida maltosa,The optimum range of pH is 6-9 for growth and4-9 for degrading phenol.The optimum temperature is 25-32℃ for growth and 28-37℃for degrading phenol.ALrirobic condition is nocessary for it to grow and degrade phenol.Adding nitrogen source can promote its growth and degradation.

关 键 词：生物降解苯酚 麦芽糖假丝酵母 环境微生物 

分 类 号：X172[环境科学与工程—环境科学]