3－硝基丙酸脂质过氧化作用的实验研究  

Effect of 3-nitroproionic acid (3-NPA)on lipid peroxidation in rats

作　　者：付以同[1] 张金松[1] 张寿林[1] 何凤生[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所

出　　处：《卫生研究》1995年第1期5-7,共3页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：９只雄性大鼠以８０ｍｇ／ｋｇ３－硝基丙酸（３－ＮＰＡ）经口染毒后１５、３０和４５ｍｉｎ时，在肝脏中用电子自旋共振仪可检测到较强的自由基信号。肾上腺素红法测定发现５×１０－３～１０－２ｍｏｌ／Ｌ３－ＮＰＡ可明显促进肝与脑微粒体和线粒体产生自由基。８０ｍｇ／ｋｇ３－ＮＰＡ灌胃染毒大鼠肝和脑中ＳＯＤ和ＧＳＨ－Ｐｘ活性上升，ＭＤＡ含量升高。因此３－ＮＰＡ在体内和体外均有诱发脂质过氧化作用的能力。The free radicals in liver of rats could be detected by electronic spintaneous resonance technique 15,30 and 45 min.After poisoning at the dosage of 80 mg/kg 3-NPA by P.O.The detection adrenochrome formation revealed that 3-NPA (5×10-3～10-2mol/L)enhanced free radical production in mitochondria and microsome of livers and brains.The activities of SOD and CHS-Px and the content of MDA increased as well in livers and brains of rats dosed with 80mg/kg 3-NDA;lt is suggested that 3-NPA is able to produce lipid peroxidation leading to the damage to the cell membrance.

关 键 词：3-硝基丙酸 脂质过氧化 鼠 动物模型 

分 类 号：R363.27[医药卫生—病理学]