合成DNA片段的分离及连接  

Separation and Ligation of Synthetic DNA Fragments

作　　者：张建文[1] 何俊[1] 王虹[1] 翟朝阳[1] 杨迪[2] 刘洪岩 杜丹萍[1] 胡凝珠[1] 郭仁[1] 

机构地区：[1]中国医学科学院医学生物学研究所 [2]复旦大学遗传研究所

出　　处：《中国医学科学院学报》1989年第3期200-204,共5页Acta Academiae Medicinae Sinicae

摘　　要：研究设计了一段带终止信号的DNA序列,通过DNA合成仪分为四个片段合成。本文介绍经DNA合成仪合成DNA片段后的处理,纯化及连接过程.经测定DNA的连接效率在65％左右。Four designed DNA fragments which contained a sequence for transcr-iptional termination were synthesized by the DNA Synthesizer Model 381 A (Applied Biosystems). The procedures for separation, purification and liga-tion of these synthetic DNA fragments were described. It was estimated that the efficiency of ligation is about 65%.

关 键 词：DNA片段 分离 连接 基因工程 

分 类 号：Q78[生物学—分子生物学]