抗人肝癌单克隆抗体重链可变区基因克隆和序列测定  

CLONING AND SEQUENCING OF IMMUNOGLOBULIN HEAVYCHAIN VAKIABLE REGION GENE OF A MONOCLONAL ANTI-BODY TO HUMAN HEPTOCARINOMA

作　　者：杨萍[1] 刘彦仿[1] 胡川闽[1] 陈志南[1] 隋延仿[1] 陈苏民[1] 高磊[1] 陈南春[1] 杨安钢[1] 

机构地区：[1]第四军医大学病理教研室,第四军医大学生化教研室

出　　处：《中国免疫学杂志》1995年第1期10-12,共3页Chinese Journal of Immunology

摘　　要：应用反转录ＰＣＲ方法从分泌抗人肝癌单克隆抗体鼠杂交瘤细胞系ＨＡｂ２７中成功地克隆了单抗重链可变区基因，将此基因重组入Ｍ１３噬菌体中，双脱氧链终止法测定了核苷酸序列。经计算机分析，基因全长４０８ｂｐ，编码１３６个氨基酸，并进行了基因同源性分析。结果表明获得的重链可变区基因是具有功能性的，两端引物自带起始码和终止码，可直接插入原核载体中进行表达。We have amplified and cloned a rearranged immunoglobulin heavy chain variable (VH)re-gion gene from the cells of a mouse hybridoma HAb27,which produce a monoclonal antibody a-gainst human hepatocarcinoma by polymerase chain reaction(PCR).The VH gene segment was408bp in length and had three complementary-determining hypervariable regions. The DNAbase sequence reavealed that the VH region gene of HAb27 cells is closely related to the myelo-ma cell line MOPC-21.

关 键 词：单克隆抗体 可变区基因 癌 肝肿瘤 克隆 序列 

分 类 号：R735.703[医药卫生—肿瘤] R730.3[医药卫生—临床医学]