麻疯树核糖体失活蛋白curcin基因的原核表达及其抗真菌活性的研究  

Prokaryotic expressed ribosome-inactivating protein(curcin)with anti-fungal activity from Jatropha curcas L.

作　　者：邵彩霞[1] 秦小波[1] 蔡峰[1] 辜小平 闫毅武 王成世[1] 徐莺[1] 陈放[1] Cai-Xia Xiao-Bo Xiao-Ping Yi-Wu Cheng-Shi 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2009年第6期1793-1797,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家“十一五”重大科技支撑计划课题(2007BAD50B00);四川省科技支撑项目(2007SZ003)

摘　　要：通过PCR方法,将麻疯树核糖体失活蛋白curcin的开放阅读框片段从麻疯树总DNA中扩增出来.并将该克隆基因片段连接到原核表达载体pQE-30中,成功构建了重组质粒pQE-C,转化大肠杆菌M15菌株.经IPTG诱导后,curcin重组蛋白在大肠杆菌中成功表达.然后通过纯化原核表达产物,得到纯化的curcin重组蛋白,并以纸片法进行体外抗菌活性检测,发现其具有较强抗真菌活性.The open reading frame (ORF)of curcin was amplified from total DNA of Jatropha curcas by PCR method.The cloned gene fragments were ligated into a prokaryotic expression vector pQE-30 and the recombination plasmid pQE-C was constructed.Then the pQE-C was transformed into E.coli M15, and the recombination curcin was expressed in M15 under IPTG induction.The purified recombination curcin was obtained and its antibacterial activity was tested in vitro by paper disk method.It was found that curcin had a better antifungal activity.

关 键 词：麻疯树核糖体失活蛋白 CURCIN 基因片段 原核表达载体 抗真菌活性 antibacterial activity expression vector 重组蛋白 转化大肠杆菌 JATROPHA curcas gene FRAGMENTS 开放阅读框 重组质粒 体外抗菌 活性检测 纯化 表达产物 IPTG诱导 in VITRO reading 

分 类 号：N55[自然科学总论]