脂肪酶催化水解反应纯化α-生育酚琥珀酸酯  

Purification of α-Tocopherol Succinate by Lipase-Catalyzed Hydrolysis

作　　者：辛嘉英[1] 吴小梅[2] 赵永杰[2] 赵贯里[1] 夏春谷[2] 李树本[2] 

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《分子催化》2005年第4期285-288,共4页Journal of Molecular Catalysis(China)

基　　金：国家自然科学基金重点项目(编号:29933040);黑龙江省自然科学基金(编号:B2004-03);哈尔滨市科学基金(编号:2004AFXXJ031);黑龙江省教育厅项目.

摘　　要：α-生育酚琥珀酸酯(α-tocopherol succinate)是天然维生素E的衍生物,它是由琥珀酸与α-生育酚的β-色酮环的6位羟基成酯而形成的化合物(图1),由于丧失了自由的羟基而不具有维生素E的抗氧化活性,在空气中较为稳定,因其可保护α-生育酚的6位羟基,使α-生育酚在储存和运输中稳定性增加,而广泛用于α-生育酚的运输和储存[1].A purified α-tocopherol succinate was obtained using an enzymatic selective hydrolysis method. β- tocopherol succinate and γ- tocopherol succinate in vitamin E succinate was selectively hydrolyzed using CRLA fraction of commercial CRI. （Candida rugosa lipase） to produce hydrophilic succinic acid and hydrophobic β- and γ- tocopherol. Removal of the β- and γ- tocopherol from lesser hydrophobic residue substrate α-tocopherol succinate with SiO2 column chromatography resulted in purification of α-tocopherol succinate. The effects of water content in the reaction medium, and reaction time on the hydrolysis conversion and purity of α-tocopherol succinate have been investigated. When the reaction time was 144 h, the hydrolysis conversion is 18. 5%, the content of α-tocopherol succinate in the residual substrate increased from 81. 6 to about 97.0%. The content of α-tocopherol succinate in the residual substrate increased slightly but the hydrolysis conversion increased obviously when the reaction time was further prolonged.

关 键 词：α-生育酚琥珀酸酯 脂肪酶 水鹪反应 纯化 

分 类 号：O643.32[理学—物理化学]