新型重组别藻蓝蛋白的高密度发酵生产和升白作用  

High cell density fermentation and leukogenic effect of a new recombinant allophycocyanin (HAPC)

作　　者：杨雨 [1] 周小龙 [2] 林凡 [1] 葛保胜 [1] 王庭健 [3] 韩璐 [1] 窦昌贵 [2] 秦松 [3] 

机构地区：[1]中国科学院海洋研究所,青岛,266071,中国科学院研究生院,北京,100049 [2]中国药科大学,南京,210038 [3]中国科学院海洋研究所,青岛,266071

出　　处：《高技术通讯》2005年第8期96-99,共4页Chinese High Technology Letters

基　　金：国家高技术研究发展计划(863计划)，中国科学院知识创新工程项目

摘　　要：在工程菌株JM109中实现了带有6×His标记的新型重组别藻蓝蛋白HAPC的高效表达.工程菌的发酵采用两步法,37℃扩增生长6h,30℃诱导培养10h.发酵液最终菌体密度(OD600)达26.25,表达蛋白占菌体总蛋白的31%.进一步用金属螯合亲和层析纯化使其纯度达90%以上.用纯化的HAPC对S180荷瘤小鼠灌胃,每天1.5～4.5mg/kg,共10天.结果表明HAPC具有明显的升白作用,可对抗环磷酰胺对免疫系统的破坏作用.A new 6 × His· tag recombinant allophycocynin HAPC was efficiently expressed in E. coli strain JM109. During the fermentation, the strain grew in 37℃ and then was induced in 30℃. After 16h fermentation, the final cell density reached OD600 26.25 with expressed HAPC to 31% of the total. The protein was purified to the purity of over 90% by metal chelate chromatograph. Stso tumor carried mice were treated with purified HAPC ig 1.5 - 4.5 mg/kg per day for 10 days. The result showed that HAPC, with obvious leukogenic effect, could inhibit immune damage of antitumor chemical cyclophosphamide （CTX）.

关 键 词：新型重组别藻蓝蛋白(HAPC) 高密度发酵 纯化 升白 别藻蓝蛋白 发酵生产 重组 高密度 工程菌株 层析纯化 

分 类 号：TQ929[轻工技术与工程—发酵工程] Q949.206[生物学—植物学]