生物素和VB_1在谷氨酸棒杆菌过量合成缬氨酸中的作用  

Effects of D-Biotin and VB_1 Factors on the Biosynthesis of Valine in a Valine Producer Corynebacterium glutamicum AATV341

作　　者：杨毅[1] 章志兰[1] 姜绪林[1] 杜大庆[1] 张星元[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《食品与生物技术学报》2005年第4期57-60,65,共5页Journal of Food Science and Biotechnology

摘　　要：通过对谷氨酸棒杆菌突变株AATV341进行生物素和VB1添加量的实验,探讨了生物素和VB1添加量对该菌株菌体质量及缬氨酸产量的影响;同时根据对AATV341进行的胞内外某些特定氨基酸和有机酸检测,研究了这两种维生素对碳架代谢流的分布,尤其是对丙酮酸节点处的代谢流量分布的影响.结果表明,生物素添加量在40μg/L时,菌体质量和缬氨酸在胞外的累积明显提高,谷氨酸在胞外累积降低;VB1的适量添加,可提高缬氨酸在胞外的累积,降低发酵液中乙酸和草酰乙酸的含量.In this paper, the effects of D-biotin and VB1 on overproduction of valine were studied, and the composition of culture medium was also optimized. The concentrations of several metabolites produced in batch cultire （extra-cellular and intra-cellular） were determined. The distributions of carbon-flux, especially at the node pyruvate were calculated, compared, and analyzed. The results showed that, in Corynebacterium glutamicum AATV341 cultivation, adding 40μg/L biotin and 400μg/L VB1 would increase the productivity of valine, and repress the extra-cellular concentrations of oxaloacetic acid, acetic acid and glutamate. The analysis may play an important role in re-designing metabolism to achieve further fermentative improvement.

关 键 词：缬氨酸 谷氨酸棒杆菌 生物素 维生素B1 碳代谢流 草酰乙酸 乙酸 

分 类 号：Q936[生物学—微生物学]